Impacts of remifentanil on the proliferation,apoptosis,and epithelial-mesenchymal transformation of lung cancer cells by regulating AKT/GSK-3β/Snail signaling pathway
Objective To investigate the impacts of remifentanil(RF)on the proliferation,apoptosis,and epithelial-mesenchymal transformation(EMT)of lung cancer cells by regulating protein kinase B(A KT)/glycogen synthase kinase-3β(GSK-3β)/Snail signal pathway.Methods Non-small cell lung cancer A549 cells were grouped into low(RF-L),medium(RF-M),high RF(RF-H)groups(10,20,40 nmol/L RF),high RF+AKT activator SC79(RF-H+SC79)group(40 nmol/L RF+4 μg/mL SC79),and control group.CCK-8 method was applied to detect cell proliferation.The cell migration ability was detected by scratch test.A transwell test was applied to detect the invasion ability of cells.The apoptosis was detected by flow cytometry.The expression levels of AKT,GSK-3β,and Snail mRNA were measured by real-time fluorescence quantitative PCR.Western blot was used to detect the ex-pression of the protein.Results Compared with the control group,the OD450 at 48 and 72 h,cell invasion numbers,wound healing rate,AKT,GSK-3β,Snail mRNA and protein expression,Bcl-2 protein,EMT-related protein N-cad-herin,and Vimentin expression were significantly lower in the RF-M and RF-H groups(P＜0.05),while the cell apoptosis rate and Bax protein,E-cadherin protein expression were significantly higher(P＜0.05).SC79 attenuated the inhibitory effect of RF on the proliferation and EMT process of lung cancer cells,and attenuated cell apoptosis.Conclusion RF can inhibit EMT and proliferation of lung cancer cells and induce apoptosis,which may be related to the inhibition of the AKT/GSK-3β/Snail signaling pathway.
remifentanilprotein kinase B/glycogen synthase kinase-3β/Snail signal pathwaylung cancerepithelial-mesenchymal transformation
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