Exploring the Relationship Between the Glymphatic System in the Healthy Brain Hemisphere and Short-Chain Fatty Acids in the Intestines:A Multi-b-value DTI-ALPS Study
Objective The purpose of this study is to quantitatively evaluate and explore the correlation between the function of the bilateral hemisphere glymphatic system in healthy individuals and the intestinalshort-chain fatty acids(SC-FAs),using the Diffusion Tensor Imaging Analysis along the Perivascular Space(DTI-ALPS)index obtained through multi-b-value data collection.Methods 29 healthy volunteers were recruited for this study,and Diffusion Spectrum Imaging(DSI)images were collected for all participants.Using FSL and itk-snap software,a circular region of interest(ROI)with a diameter of 5.0 mm was delineated at the level of the lateral ventricles in the bilateral brain hemispheres,specifically at the projection fibers and association fibers.The diffusion rates of the two types of fibers were quantitatively measured in the X,Y,and Z axes,and bilateral hemisphere DTI-ALPS indices were calculated.Stool samples were collected from all partici-pants for SCFAs analysis.Paired t-tests were conducted to compare the ALPS indices and diffusion rates of the fiber tracts in the bilateral hemispheres.Age and gender were used as covariates.Spearman partial correlation analyses were performed to examine the associations between the bilateral hemisphere ALPS indices,diffusion rates,and SCFA concentrations,with a significance level of P<0.05.Results The diffusion rate of the left-sided association fibers was higher than that of the right-sided association fibers in the Y-axis(t =2.240,P<0.05).The ALPS index of the right hemisphere was positively correlated with propionic acid(r =0.440,P =0.022).The diffusion rate of the right-sided association fibers in the X-axis was positively correlated with propionic acid(r =0.388,P =0.045).The diffusion rate of the right-sided projection fibers in the Y-axis was positively correlated with acetic acid(r =0.569,P =0.002),but negatively correlated with isobutyric acid and isovaleric acid(r =-0.458,P =0.016;r =-0.487,P =0.010).The diffusion rate of the left-sided association fibers in theX-axis wasnegatively correlated with butyric acid(r =-0.390,P =0.045).Conclusion Evaluating the func-tion of the brain glymphatic system using multi-b-value diffusion tensor MR data is feasible.There are correlations between the glymphatic system function in healthy individuals and the diffusion rates in the association and projection fiber regions,as well as the concentrations of SCFAs in the intestines.
Magnetic Resonance ImagingBrainDiffusion Tensor ImagingThe Glymphatic systemShort-ChainFatty Acids
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