Objective To observe the 64-slice spiral CT manifestations of pancreatic cystic lesions and analyze their di-agnostic criteria.Methods The clinical data of 70 patients with pancreatic cystic lesions who underwent 64-slice spiral CT examination from March 2020 to March 2023 were collected,of which 33 cases obtained diagnostic results by postopera-tive pathological examination,8 cases had puncture biopsy pathological diagnosis results,and 29 cases obtained final diag-nosis results by clinical follow-up(≥12 months).The 64-slice spiral CT diagnostic results and imaging findings of all le-sions were recorded.Results The correct diagnosis rates of pseudocysts,serous cystadenomas,mucinous cystadenomas,and solid-pseudopapillary tumors,intraductal papillary mucinous neoplasm by 64-slice spiral CT were 92.86%,81.82%,80.00%,and 83.33%,80.00%,respectively.There were statistically significant differences in the location,shape,bound-ary,cyst wall,number of cells,compartments,wall nodules or solid components,peripheral changes,calcification,pan-creatic duct dilation,and whether the five cystic lesions were interconnecting with pancreatic ducts(P<0.05).There were significant differences between serous cystadenoma and mucinous cystadenoma in morphology,maximum diameter,cyst wall,cell number and pancreatic duct dilation(P<0.05).Conclusion The internal structure of pancreatic cystic le-sions and their relationship with surrounding structures such as pancreatic ducts and blood vessels can be clearly shown by 64-slice spiral CT,which is helpful for accurate preoperative diagnosis in combination with clinical history.