Objective To investigate the value of direct lymphangiography followed by CT and 99Tcm-DX lymphography in the diagnosis of chylothorax.Methods We retrospectively analyzed the data of 51 patients who were clinically and/or pathologically diagnosed with chylothorax,and all of them underwent direct lymphangiography followed by CT and 99Tcm-DX lymphography to observe the abnormal distribution of iodine oil after direct lymphangiography,as well as abnormal distribu-tion of bilateral lower extremity drainage,thoracic duct,and jugular venous angulation area after 99Tcm-DX lymphography.Abnormalities of radiologic distribution.Results CT after direct lymphangiography showed abnormal distribution of large amounts of iodine oil in the neck,chest,abdomen and pelvis,and 99Tcm-DX lymphatic imaging showed venous angular re-gions showing,persistent and/or widened appearances showing chylothorax,chylous abdomen and celiac pericardial effu-sion,and bilaterally lower extremity slow lymphatic reflux and bilateral lower extremity lymphomas.Conclusion Direct lymphangiography followed by CT can clearly show lymphatic vessel abnormalities in different anatomical sites,whereas 99Tcm-DX lymphatic imaging functionally reacts to the leakage of chylothorax,and the combined application of the two can provide valuable imaging information for the diagnosis and differential diagnosis of this disease.