Effect of Two Kinds of Lignin on Enzymatic Hydrolysis of Cellulose
In order to investigate the effect of lignin on the enzymatic hydrolysis of cellulose, the ethanol organosolv lignins (EOL-B)and milled wood lignin(MWL-B)were extracted from bitter bamboo and added to the microcrystalline cellulose for enzymatic digestibility and adsorption.The results showed that the addition of MWL-B(8 g/L)decreased the 72 h hydrolysis yields of glucose from 51.34%to 46.06%.But the addition of EOL-B(8 g/L)significantly improved the 72 h hydrolysis yields of glucose from 51.34%to 61.06%.Langmuir adsorption isotherms showed that EOL-B had lower binding ability and maximum adsorption capacity to the cellulase enzymes than MWL-B,which led to less non-productive binding.And FT-IR and 13C NMR spectra showed that the condensed structure of C—C of lignin reduced and the β-O-4′linkages cracked in the ethanol organosolv process,which resulted in increasing the hydrophilicity of lignin,blocking the combination of lignin and the hydrophobic amino acid of cellulose,decreasing the adsorption of cellulose,increasing the cellulose concentration around the cellulose substrate and promoting the effect of enzymatic hydrolysis.
bitter bambooethanol organosolv ligninsmilled wood ligninenzymatic hydrolysishydrophilic group
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