摘要
以靛膏为原料,采用盐酸酸化、葡萄糖还原和乙酸乙酯提取3种工艺制备靛蓝酸化物、靛蓝还原物和乙酸乙酯提取物.建立高效液相色谱(HPLC)法、紫外可见分光光度(UV)法和双波长紫外分光光度(dW-UV)法,分析3种靛蓝提取物中靛蓝和靛玉红的含量,并以Kolmogorov-Smirnov检验、Bland-Altman偏差图比较和Spearman检验确定3种样品各自最佳分析方法.结果表明:所建立的HPLC法、UV法和dW-UV法简单、准确、灵敏度高、稳定性好和重复性好.3种靛蓝提取物成分含量检测方法均遵从正态分布,两两测定值存在显著差异.UV法更适于分析靛蓝酸化物、靛蓝还原物中靛蓝含量;HPLC法更适于乙酸乙酯提取物中靛蓝、靛玉红含量分析.
Abstract
Using indigo paste as raw material,indigo acidification substances,glucose reducing substances,and ethyl acetate extractives were prepared using three processes of hydrochloric acid acidification,glucose reduction,and ethyl acetate extraction,respectively.High performance liquid chromatography(HPLC),ultraviolet visible spectrophotometry(UV),and dual wavelength ultraviolet spectrophotometry(dW-UV)were established to analyze the contents of indigo and indirubin in samples from three processes.The optimal analysis methods for each of three samples were determined through Kolmogorov Smirnov test,comparison of Bland Altman deviation plots,and Spearman test.The results showed that the established HPLC,UV,and dW-UV methods were simple,accurate,highly sensitive,stable,and reproducible.Three methods for detecting the content of indigo extract components all followed the normal distribution,and there were significant differences between each the two measured values.The UV method was more suitable for analyzing the content of indigo in indigo acidified substances and indigo reducing substances.HPLC method was more suitable for measuring the content of indigo and indirubin in ethyl acetate extract.
基金项目
中央级公益性科研院所基本科研业务费专项中国林科院项目(CAFYBB2021ZC003)
贵州省科技支撑计划(黔科合支撑[2022]-159)
国家科技期刊平台
NSTL
维普
万方数据