Microalgae Embedding for Coupling Technology Research of Antibiotic Removal and Carbon Dioxide Immobilization
To address the problem of serious antibiotic pollution and excessive carbon dioxide emissions,this study utilized Chlorella pyrenoidosa as the raw material,sodium alginate as the immobilizer,and calcium chloride as the cross-linking agent to prepare C.pyrenoidosa beads.The coupled technology of antibiotic removal and carbon dioxide fixation was studied.The results indicated that when the mass fraction of sodium alginate was 2％and that of calcium chloride was 3％,the alginate beads exhibited optimal formation speed,mechanical strength,and resistance to disintegration.The presence of carbon dioxide at volume fractions of 5％and 10％significantly promoted the growth of the microalgae and enhanced its capability to process ceftriaxone.After 24 h reaction,the removal rates of Ceftriaxone were 84.89％and 84.31％,and the absorption rates of carbon dioxide were 72.25％and 71.71％.Compared with 5％and 10％volume fraction of carbon dioxide,the treatment of antibiotics and the absorption rate of carbon dioxide by C.pyrenoidosa under 20％volume fraction were significantly reduced.The activity of the carbon metabolism-related enzyme 1,5-bisphosphate ribulose carboxylase was significantly increased under 5％and 10％conditions,thereby enabling the microalgal cells to fix more carbon dioxide.The activity of carbonic anhydrase was significantly elevated under the 10％condition,facilitating the catalytic conversion of more bicarbonate into carbon dioxide,which could then be absorbed and utilized by the microalgal cells.
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