CIK增强顺铂对肺腺癌细胞系A549的杀伤作用
CIK may enhance the lethal effect of cisplatin on the lung adenocarcinoma cell line A549
李丹 1李翀 1张克新 1王智刚 1徐斌 2邓海峰 2陆明洋 2李敏 2周怡2
作者信息
- 1. 苏州大学附属第三医院呼吸内科,江苏常州213000
- 2. 苏州大学附属第三医院肿瘤生物诊疗中心,江苏常州213000
- 折叠
摘要
目的 研究细胞因子诱导的杀伤细胞(CIK)联合顺铂对人肺腺癌细胞系A549的杀伤作用.方法 在体外将健康者外周血单个核细胞(PBMC)用细胞因子诱导形成具有杀伤活性的CIK细胞.CIK、顺铂及CIK联合顺铂分别作用A549细胞24、48 h,MTT法检测细胞吸光度(A)值并计算各组细胞杀伤率.完全培养液培养CIK 48 h后,取上清液,按1∶1稀释后与A549细胞共培养24h,用流式细胞仪检测A549细胞凋亡率.结果 CIK、顺铂对A549的杀伤活性呈时间、剂量依赖性(P＜0.05).5 μg/mL顺铂联合效靶比为10∶1、20∶1的CIK共同作用A549细胞24 h,A549的杀伤率(％)分别为53.80±6.81,61.17±3.82,与单用顺铂及CIK相比,其对A549的杀伤率显著升高(P＜0.05).CIK上清液可诱导A549细胞凋亡.结论 CIK可增强顺铂对A549细胞系的杀伤作用.
Abstract
Objective To investigate the lethal effect of cytokine-induced killer cells (CIK) combined with cisplatin on the human lung adenocarcinoma cell line A549.Methods The peripheral blood mononuclear cells (PBMC) from healthy persons were induced to be the CIK cells with cytotoxicity by cytokines in vitro.Then,A549 cells were incubated with CIK,cisplatin and CIK combined with cisplatin for 24 and 48 hours,respectively.The kill ratios of A549 cells were detected by the MTT assay.After CIK cells were incubated with complete culture medium for 48 h,the supernatant was collected and diluted 1 ∶ 1 with medium,and then incubated with A549 cells for 24 h.Next,the apoptosis rate of A549 cells was detected by flow cytometry.Results Both CIK and cisplatin killed A549 cells in a dose-and time-dependent manner (P ＜ 0.05).After A549 cells were incubated with 5 μg/mL of cisplatin combined with CIK with 10∶1 and 20∶1 of effector-target ratios for 24 h,the kill ratios of A549 cells were (53.80 ± 6.81)％ and (61.17 ± 3.82) ％,respectively,which were significantly higher than that of cisplatin or CIK alone (P ＜ 0.05).The supernatant of CIK cells could induce the apoptosis of A549 cells.Conclusion CIK may enhance the lethal effect of cisplatin on the lung adenocarcinoma cell line A549 in vitro.
关键词
肺肿瘤/肺腺癌细胞株/细胞因子诱导的杀伤细胞/顺铂Key words
lung cancer/lung adenocarcinoma cell line/cytokine-induced killer cell/cisplatin引用本文复制引用
基金项目
出版年
2013
NETL
NSTL
维普
万方数据