不同碘水平下甲状腺结节患者血清外泌体中miRNA生物学标志物的筛选
Screening of miRNA biomarkers in serum exosomes of patients with thyroid nodules at different iodine levels
曹文源 1赵鸿渐 2邢浩 3张辉 4孔伟 5刘庆华 6尹峰燕 3何倩 7邢薇佳7
作者信息
- 1. 山东第一医科大学公共卫生与健康管理学院,研究生院,济南 250000
- 2. 成武县人民医院肝胆外科,山东成武 274200
- 3. 泰安市中心医院甲状腺外科,山东泰安 271000
- 4. 泰安市中心医院检验科,山东泰安 271000
- 5. 山东第一医科大学第二附属医院甲状腺外科,山东泰安 271000
- 6. 山东第一医科大学第二附属医院检验医学科,山东泰安 271000
- 7. 山东第一医科大学公共卫生与健康管理学院,流行病学教研室,济南 250000
- 折叠
摘要
目的 比较不同碘水平下甲状腺结节患者与健康人血清外泌体微小核糖核酸(microRNA,miRNA)表达谱的差异,分析其共同点,为筛选不同碘水平下甲状腺结节早期诊断标志物提供参考依据.方法 收集 10 例不同碘水平下甲状腺结节患者及体检健康者的外周血样本,采用砷铈催化分光光度法测定各组血清碘水平;提取外泌体miRNA,利用高通量测序技术测定血清miRNA的表达水平;预测差异靶基因,并进一步进行GO(Gene ontology)分析和KEGG(Kyoto Encyclopedia of Genes and Genomes)分析.结果 在不同碘水平的甲状腺结节患者与健康人群中存在 6 个表达下调的 miRNA,分别为 miR-324-5p、miR-6511b-3p、miR-9903、miR-550a-3p、miR-5001-3p、miR-3688-3p.差异表达的外泌体 miRNA 可调控 MAPK 信号通路、PI3K-AKT信号通路、VEGF 信号通路及NF-κB信号通路.结论 筛选出 6 个差异表达miRNA,有望作为不同碘水平下甲状腺结节早期诊断的生物学标志物.
Abstract
Objective To comapre and analyze the differences and commonalities of expression profiles of serum exosomal microRNA between patients with thyroid nodules and healthy persons at different iodine levels,and then provide evidence for screening early diag-nostic markers of thyroid nodules at different iodine levels.Methods The peripheral blood samples from 10 patients with thyroid nod-ules and healthy volunteers at different iodine levels were collected.Their serum iodine levels were measured by the arsenic cerium cat-alytic spectrophotometry.Serum exosomal microRNA were extracted and the expression levels of microRNA were determined by the high-throughput sequencing technology.The differential target genes were predicted and further performed Gene ontology(GO)analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis.Results Compared with healthy volunteers,there were 6 downreg-ulated miRNAs in the patients with thyroid nodules at different iodine levels,namely miR-324-5p,miR-6511b-3p,miR-9903,miR-550a-3p,miR-5001-3p,and miR-3688-3p.Differentially expressed exosomal microRNA could regulate the MAPK signaling path-way,PI3K-AKT signaling pathway,VEGF signaling pathway,and NF-κB signaling pathway.Conclusion Six differentially expressed microRNAs is identified,which may serve as biological markers for the early diagnosis of thyroid nodules at different iodine levels.
关键词
甲状腺结节/碘/外泌体/microRNAKey words
thyroid nodule/iodine/exosome/microRNA引用本文复制引用
基金项目
山东省自然科学基金(ZR2023QH135)
出版年
2024
NETL
NSTL
维普
万方数据