Predictive values of the combination detection of plasma miR-320c,miR-126 and miR-34c in the severity of chronic obstruc-tive pulmonary disease
Objective To investigate the correlation between plasma miRNA levels and the severity of chronic obstructive pulmonary disease(COPD).Methods Quantitative fluorescent PCR(qPCR)was employed to analyze plasma miRNAs in a cohort comprising 10 healthy subjects and 10 COPD patients.Subsequently,miRNAs closely associated with COPD were identified.Additionally,plasma samples from healthy adults(n=28),COPD patients with GOLD gradeⅠ(n=30),gradeⅡ(n=32),gradeⅢ(n=30),and gradeⅣ(n=28)were utilized to select candidate miRNAs.The roles of these candidate miRNAs,both individually and in combination,in pre-dicting COPD severity were validated.Results The levels of plasma miR-320c,miR-126,and miR-34c were found to be significantly elevated in COPD patients compared to healthy individuals(P＜0.01).Furthermore,the expression abundance of these miRNAs showed an increasing trend with higher COPD grades.The areas under the receiver operating characteristic(ROC)curve of miR-320c,miR-126,miR-34c,and their combination for predicting COPD severity were 0.822,0.705,0.783,and 0.892,respectively.Conclu-sion The levels of plasma miR-320c,miR-126,and miR-34c can predict the severity of COPD,and their combination detection ex-hibits significantly higher predictive efficacy than single miRNA analysis.
NETL
NSTL
万方数据
维普
