摘要
目的 探讨血浆miRNA水平与慢性阻塞性肺疾病(COPD)严重程度的相关性.方法 利用荧光定量PCR(qPCR)检测健康人群(10 例)及COPD患者(10 例)血浆miRNA的表达水平,筛选与COPD患者密切相关的miRNA.进一步在健康人群(28 例)和COPD慢性阻塞性肺疾病全球创议(GOLD)Ⅰ级(30 例)、Ⅱ级(32 例)、Ⅲ级(30 例)及Ⅳ级(28 例)患者中明确候选miRNA单独及联合检测对COPD严重程度的预测价值.结果 COPD患者血浆miR-320c、miR-126 及miR-34c水平显著高于健康人群(P＜0.01).随着COPD级别的增加,血浆miR-320c、miR-126 及miR-34c的表达水平亦增加.miR-320c、miR-126、miR-34c单独及联合检测预测中重度COPD患者的ROC曲线下面积(AUCROC)分别为 0.822、0.705、0.783及 0.892.结论 血浆miR-320c、miR-126 及miR-34c可预测COPD严重程度,其联合检测的预测效能显著高于单项miRNA.
Abstract
Objective To investigate the correlation between plasma miRNA levels and the severity of chronic obstructive pulmonary disease(COPD).Methods Quantitative fluorescent PCR(qPCR)was employed to analyze plasma miRNAs in a cohort comprising 10 healthy subjects and 10 COPD patients.Subsequently,miRNAs closely associated with COPD were identified.Additionally,plasma samples from healthy adults(n=28),COPD patients with GOLD gradeⅠ(n=30),gradeⅡ(n=32),gradeⅢ(n=30),and gradeⅣ(n=28)were utilized to select candidate miRNAs.The roles of these candidate miRNAs,both individually and in combination,in pre-dicting COPD severity were validated.Results The levels of plasma miR-320c,miR-126,and miR-34c were found to be significantly elevated in COPD patients compared to healthy individuals(P＜0.01).Furthermore,the expression abundance of these miRNAs showed an increasing trend with higher COPD grades.The areas under the receiver operating characteristic(ROC)curve of miR-320c,miR-126,miR-34c,and their combination for predicting COPD severity were 0.822,0.705,0.783,and 0.892,respectively.Conclu-sion The levels of plasma miR-320c,miR-126,and miR-34c can predict the severity of COPD,and their combination detection ex-hibits significantly higher predictive efficacy than single miRNA analysis.
基金项目
上海市金山区科学技术创新资金项目(2021-3-06)
NETL
NSTL
维普
万方数据