Clinical significance of the detection of SORT1 gene in peripheral blood mononuclear cells for the diagnosis of pediatric sepsis
Objective To explore the clinical significance of sortilin 1(SORT1)gene as a new diagnostic biomarker for pediatric sep-sis.Methods Three pediatric sepsis-related microarray datasets,including GSE13904,GSE26378,and GSE26440,were obtained from the gene expression omnibus(GEO)database to analyze the expression difference of SORT1 gene between normal and sepsis sam-ples.Then,65 clinical samples were used to validate the difference through qRT-PCR.The molecular mechanism and biological process associated with SORT1 gene were explored by the gene set enrichment analysis(GSEA).Results The expression levels of SORT1 gene in the sepsis samples of GSE13904,GSE26378,and GSE26440 datasets and clinical samples were higher than that in normal samples.Compared with commonly used inflammatory markers such as procalcitonin(PCT),C-reactive protein(CRP),white blood cell(WBC),and the percentage of neutrophils(Neu％),SORT1 gene showed better diagnostic ability for pediatric sepsis and the area under the receiver operating characteristic(ROC)curve(AUCROC)was 0.863.SORT1 gene may be involved in the occurrence and de-velopment of pediatric sepsis through the TNF-α/NF-κB signaling pathway.Conclusion SORT1 gene may have certain diagnostic val-ue for pediatric sepsis,which needs to be further confirmed in clinical practice.
pediatric sepsissortilin 1 genereceiver operating characteristic curvegene set enrichment analysis
万方数据
维普
