Application value of HBV DNA load and serological markers in the prevention and treatment of children's hepatitis B
Objective To analyze the fluorescence quantitative detection results of hepatitis B virus(HBV)DNA and distribution characteristics of serological markers in children aged 16 and below in Wuxi area,and provide evidence for improving the prevention and control measures of HBV in children.Methods The HBV DNA load in the serum of pediatric patients was detected by fluores-cence quantitative PCR,and the serological markers and liver function indexes of HBV were detected by the chemiluminescence and automatic biochemical analyzer methods,respectively.The data were analyzed using SPSS 13.0 statistical software.Results Among the 86 children who underwent fluorescence quantitative detection of HBV DNA,45 were detected for HBV DNA,with a positive rate of 52.3％,of which 34 had high viral load.Among the children with positive HBV DNA,35 were boys and 10 were girls.There was no statistical significance in the positive rate of HBV DNA between boys and girls(x2=3.688,P=0.055).There was a statistically signif-icant difference in the positive rate of HBV DNA among different age groups(x2=5.540,P=0.019),and the highest positive rate was found in children aged 15.A total of 64 pediatric patients were tested for the serological antigen and antibody markers of HBV,and 9 antigen-antibody combination patterns were found.A total of 80 pediatric patients were tested for liver function indexes,and 33 of them were found to have at least one abnormal index.Conclusion The detection of HBV DNA by fluorescence quantitative PCR comple-ments the detection of serological markers and biochemical indexes,and their combination can improve the accuracy of clinical diagno-sis of hepatitis B in children,which may provide effective reference for clinical judgment of the degree and infectivity of HBV infection.
hepatitis B viruschildrenfluorescence quantitative PCRviral loadserological marker
万方数据
维普
