miR-548d-5p contributes to cell proliferation and osteogenic differentiation via TLR2/Myd88/NF-κB axis in periodontal ligament stem cells
Objective:To explore the effect and mechanism of TLR2 negative upstream regulatory factor miR-548d-5p on the proliferation and osteogenic differentiation of human periodontal ligament stem cells(hPDLSCs).Methods:Construct TLR2 overexpression vector TLR2 OE.TLR2 OE and miR-548d-5p mimic were transfected into hPDLSCs respectively.The transfection efficiency was evaluated by RT-qPCR.Proliferation ability of hPDLSCs was detected by CCK-8 assay and clone formation experiment.Alizarin red staining and Western blotting were used to determine the expression level of OCN protein and evaluate the osteogenic differentiation ability of hPDLSCs.TLR2/MyD88/NF-κB signaling pathway molecules Myd88 and NF-κB expression levels were determined by Western blot.Results:The survival rate,clone formation numbers and num-bers of mineralized nodules formed by hPDLSCs were significantly reduced after transfection with TLR2 OE,while the ex-pression levels of Myd88 and NF-κB molecules were significantly incresed(P＜0.05).The survival rate,clone formation num-bers and numbers of mineralized nodules formed by hPDLSCs were significantly incresed after transfection with miR-548d-5p mimic,while the expression levels of Myd88 and NF-κB molecules were significantly reduced(P＜0.05).Compared with the control group,there was no significant difference in survival rate,clone formation numbers,numbers of mineralized nodules formed by hPDLSCs and Myd88 and NF-κB expression levels in TLR2 OE and miR-548d-5p mimic co-transfection group.Conclusion:Overexpression of TLR2 inhibited the proliferation and osteogenic differentiation of hPDLSCs.miR-548d-5p en-hanced the proliferation and osteogenic differentiation ability of hPDLSCs.MiR-548d-5p could antagonize the effect of TLR2 on the proliferation and osteogenic differentiation of hPDLSCs through regulating TLR2/Myd88/NF-κB signaling pathway.
Toll like receptor 2Periodontal ligament stem cellsmiRNA
国家科技期刊平台
NSTL
万方数据
维普
