Screening and efficacy evaluation of oral squamous cell carcinoma based on quantitative analysis of DNA ploidy in exfoliated cells
Objective:To explore the application and effectiveness evaluation of DNA ploidy quantitative analysis technology in screening oral squamous cell carcinoma.Methods:From March 2022 to June 2023,DNA ploidy screening and liquid based cytology examinations were performed on 107 outpatient and inpatient patients at the Stomatological Hospital of Xi'an Jiaotong University.Patients with DI>2.5 were considered positive and undergo pathological tissue biopsy,with DI<2.5 considered negative if there are no clear clinical malignant indications.The results of DNA ploidy screening were com-pared with the results of liquid based cytology and pathological biopsy.Results:Am ong the 107 patients,28 underwent path-ological examination,and 27 of them had DNA ploidy diagnosis opinions that were consistent with the pathological report re-sults,with a coincidence rate of 96.43％.A total of 24 patients with DI>2.5 and pathological results were malignant.The di-agnostic opinion of DNA ploidy was consistent with the pathological report results,with a coincidence rate of 100％.One case with DI>2.5 and no clear malignancy in pathological results was squamous papillary hyperplasia,with some epithelial moder-ate to severe atypical hyperplasia.Two patients with DI<2.5 and pathological results as benign,the pathological result showed 1 patient with malignancy,indicating effective detection of insufficient cells.There were total of 22 cases of oral squamous cell carcinoma,and the diagnostic opinion of DNA ploidy in 21 cases were consistent with the pathological report results,with a coincidence rate of 95.45％.Conclusion:For oral squamous cell carcinoma,the coincidence rate between DNA ploidy screening results and pathological biopsy results is high.DNA ploidy quantitative analysis is more sensitive than traditional cytological examinations.It is a reliable and objective non-invasive screening method for oral squamous cell carcinoma.
DNA ploidy quantitative analysis technologyOral Squamous Cell CarcinomaScreening
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