Effect of dioscin on proliferation and apoptosis of oral squamous cell carcinoma cells
Objective:To investigate the effect of dioscin on the proliferation and apoptosis of human oral squamous cell carcinoma cells SCC-4 and SCC-25.Methods:SCC-4 and SCC-25 cells were treated with different concentrations(0,0.1,0.3,1,3,10,30 µmol/L)of dioscin for 24 h,48 h and 72 h.The effect of dioscin on the proliferation of SCC-4 and SCC-25 cells was detected by CCK-8 method,and the subsequent experimental concentrations(0,1,2,4 µmol/L)were select-ed according to the IC50 value.EdU was used to detect the effect of dioscin on the proliferation of SCC-4 and SCC-25 cells.The effect of dioscin on the apoptosis of SCC-4 and SCC-25 cells were detected by flow cytometry.The expression levels of Noxa and Cleaved-Caspase 3 proteins in cells treated with dioscin for 48 h were detected by Western Blot.The mRNA expres-sion of Noxa in cells was detected by qRT-PCR.Results:Within a certain concentration range,dioscin could inhibit the pro-liferation of oral squamous cell carcinoma cells SCC-4 and SCC-25 in a time-dependent and dose-dependent manner(P＜0.05).At the same time,the experimental group(1,2,4 μmol/L)dioscin could promote the apoptosis of SCC-4 and SCC-25 cells after 48 h intervention(P＜0.05),and up-regulate the expression levels of Noxa and Cleaved-Caspase 3 in SCC-4 and SCC-25 cells,and the mRNA expression of Noxa in SCC-4 and SCC-25 cells increased with the concentration(P＜0.05).Conclusion:A certain concentration of dioscin can effectively inhibit the proliferation of oral squamous cell carcinoma cells and induce the apoptosis of oral squamous cell carcinoma cells.The mechanism may be related to the up-regulation of the ex-pression of pro-apoptotic protein Noxa by dioscin.
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