The mechanism of BMSC Exosomes inhibiting Rho/ROCK signaling pathway on the biological activity of oral cancer cells
Objective:To investigate the mechanism of inhibition of Rho/ROCK signal pathway by Exosomes of bone marrow mesenchymal stem cell(BMSC)on the biological activity of oral cancer cells.Methods:Cell groups included control group:normal fetal bovine serum cultured Tca8113 cells.In the Exosomes group,Tca8113 cells were co-cultured with 1 μmol/L Dil labeled with Exosomes.Y27632 group:Tca8113 cells were co-cultured with Rho/ROCK signaling pathway in-hibitor Y27632.Combination group:Tca8113 cells were co-cultured with Exosomes+Y27632.The cell activity was detected by CCK-8.The apoptosis rate was detected by flow cytometry.Cell migration distance was detected by scratch test.The number of cell invasion was detected by Transwell method.The expression of RhoA,RhoC,ROCK1 and ROCK2 were detected by West-ern blot.Results:Compared with the control group,both the apoptosis rate and migration distance and the number of cell in-vasion are decreased at 24,48,72,and 96 hours,with no statistically significant difference between the Exosomes group and Y27632 group.Compared with Y27632 group,the cell activity,cell migration distance and invasion number in combination group were decreased(P＜0.05).Compared with the control group,RhoA,RhoC,ROCK1,and ROCK2 in Tca8113 cells were decreased in Exosomes group(P＜0.05).Compared with Y27632 group,RhoA,RhoC,ROCK 1 and ROCK2 in combination group were decreased(P＜0.05).Conclusion:Exosomes of BMSC can increase apoptosis,inhibit cell activity,invasion and migration of oral cancer Tca8113 cells.The mechanism may be related to inhibition of Rho/ROCK signal pathway activation.
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