LncRNA-TNFRSF13C regulates the effects of miR-1246 on HIF-1α and bioactivity of LPS-induced dental pulp cells
Objective:To investigate the effect of LncRNA-TNFRSF13C regulation of miR-1246 on hypoxia-inducible factor 1α(HIF-1α)and bioactivity of lipopolysaccharides(LPS)-induced dental pulp cells.Methods:After human dental pulp cells(HDPCs)treated with LPS,they were divided into HDPCs group,TNFRSF13C NC-siRNA group,TNFRSF13C-siRNA group,sno-TNFRSF13C group,miR-NC group,miR-1246 mimics group,and miR-1246 siRNA group,TNFRSF13C-siRNA+miR-1246 siRNA group.The relative expressions of LncRNA-TNFRSF13C and miR-1246 were detected by qRT-PCR.The expression of HIF-1α was detected by Western blotting.CCK-8 was used to detect cell activity in each group.The apoptosis rate was detected by flow cytometry.The mineralization of cells was observed by alizarin red staining.Results:Com-pared with HDPCs group and TNFRSF13C NC-siRNA group,HIF-1α expression,apoptosis rate and LncRNA-TNFRSF13C expression in TNFRSF13C-SiRNA group were significantly decreased,and cell proliferation rate was significantly increased(P<0.05).Compared with miR-NC group,HIF-1α expression,cell apoptosis rate and expression level of miR-1246 in miR-1246 siRNA group were significantly decreased,and cell proliferation rate was significantly increased(P<0.05).Compared with TNFRSF13C-siRNA group and miR-1246 siRNA group,HIF-1α expression,cell apoptosis rate in TNFRSF13C-siRNA+ miR-1246 siRNA group were significantly decreased,cell proliferation rate was significantly increased(P<0.05).TN-FRSF13C-siRNA group and miR-1246 siRNA group had more mineralized nodules and dense mass.The TNFRSF13C-siRNA+ miR-1246 siRNA group had a large number of mineralized nodules,which were dense and deeply stained.Conclusion:Inhi-bition of LncRNA-TNFRSF13C can promote the activity of LPS-treated HDPCs,reduce the effect of apoptosis,promote the mineralization of HDPCs,and inhibit HIF-1α.The study mechanism is related to the regulation of miR-1246 activity.
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