首页|miR-2355-3p通过靶向SERPINA3增强口腔鳞状细胞癌细胞的放射敏感性

miR-2355-3p通过靶向SERPINA3增强口腔鳞状细胞癌细胞的放射敏感性

扫码查看
原文链接
  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
目的:探讨miR-2355-3p对口腔鳞状细胞癌放射敏感性的影响及其机制.方法:回顾性收集20例口腔鳞状细胞癌和20例正常口腔黏膜组织,qRT-PCR法检测miR-2355-3p的表达,分析miR-2355-3p表达与患者临床病理特征的关系.利用TCGA数据分析miR-2355-3p在口腔鳞状细胞癌中的表达、预后及诊断价值.qRT-PCR检测细胞中miR-2355-3p、丝氨酸蛋白酶抑制剂家族A成员3(serpin family A member 3,SERPINA3)的相对表达情况,CCK-8、EdU和流式细胞术检测细胞活力、增殖和凋亡.TargetScan 8.0预测miR-2355-3p潜在的下游靶基因及结合位点.双荧光素酶报告基因实验验证miR-2355-3p与SERPINA3的相关性.qRT-PCR和Western blot检测FADU细胞中SERPINA3 mRNA和蛋白的表达.结果:miR-2355-3p在口腔麟状细胞癌中高表达.高水平miR-2355-3p的口腔鳞状细胞癌患者预后较差.miR-2355-3p预测口腔鳞癌的AUC为0.864(95％CI:0.798～0.931).miR-2355-3p inhibitor下调miR-2355-3p表达.电离辐射(ionizing radiation,IR)处理抑制FADU细胞活性和增殖,促进细胞凋亡.转染miR-2355-3p inhibitor后,miR-2355-3p inhibitor进一步抑制FADU细胞活性和增殖,促进细胞凋亡.miR-2355-3p 可直接靶向 SERPINA3.miR-2355-3p inhibitor 促进 FADU 细胞 SERPINA3 mRNA 和蛋白的表达.结论:miR-2355-3p通过靶向SERPINA3增强口腔鳞状细胞癌细胞的放射敏感性.
MiR-2355-3p enhances radiosensitivity of oral squamous cell carcinoma cells by targeting SERPINA3
Objective:To investigate the effect and mechanism of miR-2355-3p on radiosensitivity of oral squamous cell carcinoma(OSCC).Methods:Retrospectively collect 20 cases of OSCC and 20 cases of normal oral mucosal tissue.The miR-2355-3p expression was detected using qRT-PCR,and the clinical pathological characteristics of patients were analyzed.Using TCGA data to analyze the expression,prognosis and diagnostic value of miR-2355-3p in OSCC.qRT-PCR detected the relative expression of miR-2355-3p and serpin family A member 3(SERPINA3)in FADU cells,CCK-8,EdU,and flow cytom-etry were used to detect the cell viability,proliferation,and cell apoptosis.TargetScan 8.0 predicted the potential downstream target genes and binding sites of miR-2355-3p.Dual-luciferase reporter assay validated the correlation between miR-2355-3p and SERPINA3.qRT-PCR and Western blot detected the relative expression of SERPINA3 mRNA and protein in FADU cells.Results:miR-2355-3p was significantly upregulated in OSCC,indicating a prognosis for OSCC patients with high levels of miR-2355-3p.The AUC of miR-2355-3p in predicting OSCC was 0.864(95％CI:0.798～0.931)for OSCC.Inhibition of miR-2355-3p led to downregulation of miR-2355-3p expression in FADU cells.Ionizing radiation(IR)treatment suppressed cell activity and proliferation while stimulated cell apoptosis of FADU cells.Transfection with miR-2355-3p inhibitor further inhibited cell viability and proliferation,while increased apoptosis of FADU cells.miR-2355-3p directly targeted SERPINA3.Inhibition of miR-2355-3p promoted the expression of SERPINA3 mRNA and protein in FADU cells.Conclusion:miR-2355-3p enhanced the radiosensitivity of oral squamous cell carcinoma cells by targeting SERPINA3.

Oral squamous cell carcinomamiR-2355-3pSERPINA3Cell proliferationRadiosensitivity

陆开睿、潘树矿、胡梦甜、丁凤、王育新、薛洪宝

展开 >

蚌埠市第三人民医院口腔科 安徽 蚌埠 233000

南京市口腔医院口腔颌面外科 江苏 南京 210000

蚌埠医科大学公共基础学院 安徽 蚌埠 233000

口腔鳞状细胞癌 miR-2355-3p SERPINA3 细胞增殖 放射敏感性

安徽省高校学科(专业)拔尖人才学术资助项目安徽省新时代育人质量工程研究生教育项目安徽省新时代育人质量工程研究生教育项目

gxbjZD20220412022lhpysfjd0612022sshqygzz033

2024

10.3969/j.issn.1003-1634.2024.07.002

临床口腔医学杂志
华中科技大学同济医学院附属同济医院,中华口腔医学会口腔黏膜病专业委员会 中华医学会武汉分会

临床口腔医学杂志

CSTPCD
影响因子：0.783
ISSN：1003-1634
年,卷(期)：2024.40(7)