In vitro study of 3D printed gelatin/bone powder scaffold promoting osteogenesis differentiation
Objective:To prepare a composite bioink from methylacry lated gelatin(GelMA)and Bio-Oss.The Bio-Oss/GelMA-loaded bone marrow mesenchymal stem cell(BMSCs)composite bone tissue engineering scaffold was constructed by 3D bioprinting to explore its biocompatibility and in vitro osteogenic differentiation ability.Methods:GelMA/Bio-Oss/BMSCs were prepared by 3D bioprinting by adding different concentrations of Bio-Oss in GelMA.The best suitable concentra-tion of GelMA/Bio-Oss/BMSCs was determined by electron microscopy,live/dead staining,cell proliferation experiments,al-kaline phosphatase(ALP)staining,and RT-qPCR.Results:Bone tissue engineering scaffold structure was stable.The added of Bio-Oss showed no obvious toxicity on cell viability culture both during and after printing.High concentration Bio-Oss group promoted the expression of osteogenic genes OPN,RUNX2,OCN and enhanced ALP activity significantly.Conclusion:GelMA/Bio-Oss/BMSCs composite scaffolds has good biocompatibility and has an obvious effect in promoting the osteogenic differentiation of BMSCs.
3D bioprintingGelatin methacryloylBone marrow mesenchymal stem cellsOsteogenesis differentia-tionBone regenerationBone defect
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