Galectin-1蛋白在肾透明细胞癌组织中的表达及患者预后判断中的价值
Expression and prognostic value of Galectin-1 protein in clear cell renal cell carcinoma
常晨 1毕新刚 2常乐3
作者信息
- 1. 陕西省人民医院病理科(西安,710068)
- 2. 国家癌症中心/国家肿瘤临床医学中心/中国医学科学院北京协和医学院肿瘤医院泌尿外科
- 3. 陕西省人民医院陕西省感染与免疫疾病重点实验室;陕西省人民医院陕西省细胞免疫工程技术研究中心
- 折叠
摘要
目的:探讨肾透明细胞癌中半乳糖凝集素-1(Galectin-1)蛋白的表达变化,并评估其在患者预后判断方面的潜在价值.方法:采用组织微阵列和免疫组织化学技术对173例肾透明细胞癌组织及其相应的手术切缘正常组织中Galectin-1蛋白表达水平进行检测.收集患者的随访信息,并分析其表达水平与临床病理参数以及患者预后的相关性.结果:免疫组织化学染色结果显示,在173例肾透明细胞癌组织中,28.9％(50/173)的患者Galectin-1蛋白表达水平升高,而在癌旁正常组织中无表达,两者比较差异有统计学意义(P＜0.01).统计分析结果表明,在肾透明细胞癌组织中,Galectin-1蛋白表达水平与T分期、N分期、M分期、坏死区域存在、侵犯肾盂、伴肉瘤样分化以及临床分期均呈显著正相关(均P＜0.05).同时,Galectin-1蛋白的表达水平与肾透明细胞癌患者的预后密切相关.Kaplan-Meier生存曲线显示高表达患者的生存时间明显短于低表达患者(P＜0.05).经过多因素Cox回归分析的进一步验证,Galectin-1的高表达被确认为肾透明细胞癌患者不良预后的独立预测因素.结论:本研究发现肾透明细胞癌组织中Galectin-1蛋白表达水平升高,该高表达与患者预后不良相关,提示Galectin-1有可能成为肾透明细胞癌预后评估的潜在分子标志.
Abstract
Objective:To examine alterations in the expression of Galectin-1 protein in clear cell renal cell car-cinoma(ccRCC)and assess its potential significance for predicting patient prognosis.Methods:The Galectin-1 protein expression was assessed using tissue microarrays and immunohistochemistry in surgically-resected tumor tissues and adjacent normal cells obtained from 173 patients with ccRCC.Follow-up data on the patients were gathered,and the analysis involved examining the correlation between Galectin-1 expression levels and clinicopath-ological parameters,as well as assessing its association with patient prognosis.Results:Immunohistochemical staining results showed that Galectin-1 protein was highly expressed in 28.9％(50/173)of ccRCC,while there was no expression in the adjacent normal tissues.There was a significant difference between the two groups(P＜0.01).The statistical analysis results indicate that the protein expression level of Galectin-1 in ccRCC is signifi-cantly positively correlated with T stage,N stage,M stage,presence of necrotic areas,invasion of the renal pel-vis,sarcomatoid differentiation,and clinical stage(P＜0.05).Simultaneously,the protein expression level of Ga-lectin-1 is closely associated with the prognosis of patients with ccRCC.Kaplan-Meier survival curves indicate that the survival time of patients with high expression is significantly shorter than that of patients with low expression(P＜0.05).Further validation through multifactorial Cox regression analysis confirms that the high expression of Galectin-1 is identified as an independent predictor of adverse prognosis in patients with ccRCC.Conclusion:The expression of Galectin-1 protein elevated in ccRCC,suggesting its potential role as a molecular marker for anticipa-ting an unfavorable prognosis in patients with ccRCC.
关键词
肾透明细胞癌/免疫组织化学/半乳糖凝集素-1/预后标志物/总生存率Key words
clear cell renal cell carcinoma/immunohistochemical/Galectin-1/prognostic marker/overall survival rate引用本文复制引用
基金项目
陕西省自然科学基金(2023-JC-QN-0851)
陕西省人民医院科技人才支持计划(2022JY-44)
陕西省人民医院科技人才支持计划(2023JY-08)
中国医学科学院医学与健康科技创新工程(2021-12M-1-033)
出版年
2024
NETL
NSTL
万方数据