Objective:To explore the significance of B-ultrasonography combined with color Doppler ultrason-ic-guided puncture for reducing bleeding and postoperative hospital stay in percutaneous nephrolithotomy(PCNL).Methods:The clinical data of 120 patients with renal calculi and/or upper ureteral calculi who underwent PCNL from June 2021 to June 2023 in Tongren Hospital,Shanghai Jiao Tong University School of Medicinewere retro-spectively analyzed.According to the different guiding puncture methods,they were divided into a simple B-ultra-sound gray-scale scan-guided puncture group and B-ultrasound combined with color Doppler-guided puncture group.Surgical complications,especially surgical bleeding and postoperative hospital stay were compared between the two groups.Results:There was no significant difference between the two groups(P>0.05)in age([54.110± 11.218]years vs[53.001±12.575]years),stone size([622.074±59.433]mm2 vs[678.125±52.834]mm2),op-eration time([87.483±21.558]min vs[86.217±24.399]min),operation channel([1.150±0.360]vs[1.183± 0.390]),preoperative hemoglobin([136.651±15.713]g/L vs[135.301±13.982]g/L)or postoperative hemo-globin([123.533±14.623]g/L vs[126.166±13.176]g/L).In the comparison of postoperative hospital stay([7.250±1.997]days vs[6.150±1.176]days)and hemoglobin decrease([13.117±9.106]g/L vs[9.133± 6.588]g/L),there were statistical significant differences between the two groups(P<0.05).Conclusion:B-ultra-sound combined with color Doppler guided puncture can monitor and avoid large blood vessels in the kidney in real time,so it can significantly reduce PCNL bleeding and postoperative hospitalization days.