Discovery of novel biomarkers and pathways in aldosterone-producing adenomas through label-free quantitative proteomics analysis
Objective:To identify differentially expressed proteins between aldosterone-producing adenoma(APA)tissue and normal adrenal tissue through label-free quantitative proteomics analysis of APA tissue collected from patients undergoing adrenal resection.Methods:The APA and normal adrenal tissue were analyzed using la-bel-free quantitative proteomics analysis to identify differentially expressed proteins.Immunohistochemical stai-ning was used to validate the expression differences of the identified proteins between APA and normal tissue in patient samples.Results:A total of 6282 proteins were identified.Using a significance cutoff of P＜0.05 and|fold change|≥ 2 356 differentially expressed proteins were identified.Furthermore,through the integration of proteomics and transcriptomics data,a novel biomarker,chromogranin B(CHGB),was identified with significant-ly downregulated expression in APAs,which was further validated through immunohistochemical staining.ROC curve analysis showed that CHGB had high specificity in distinguishing normal tissues from APAs(AUC=0.856),indicating its potential importance in APA development.Importantly,the combination of CHGB and CYP11B2 improved the predictive efficiency for diagnosing APAs.Conclusion:Our study identified key proteins and pathways involved in APA development and discovered a novel protein biomarker for distinguishing APAs,which may serve as a potential biomarker for predicting APAs.These findings provide a theoretical basis for fu-ture molecular diagnostic research in APAs.
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