血清miR-296-3p和miR-324-3p表达与肾癌患者临床病理特征及预后的关系
Relationship between serum miR-296-3p and miR-324-3p expression and clinical pathological features and prognosis of renal cell carcinoma patients
兰东 1郭军宏 2袁文强 1杜建辉1
作者信息
- 1. 广安市人民医院泌尿外科(四川广安,638500)
- 2. 深圳市宝安区松岗人民医院泌尿外科
- 折叠
摘要
目的:分析血清微小核糖核酸-296-3p(microribonucleic acid-296-3p,miR-296-3p)和微小核糖核酸-324-3p(microribonucleic acid-324-3p,miR-324-3p)表达水平与肾癌患者临床病理特征及预后的关系.方法:选择2017年1月-2020年12月广安市人民医院收治的90例肾癌患者(肾癌组),另选择同时期本院收治的90例肾良性病变患者(良性组),同时期来院接受体检的90例健康体检者(健康组);采用实时荧光定量PCR(RT-PCR)法检测血清中miR-296-3p和miR-324-3p的相对表达量;采用Cox回归分析影响肾癌患者预后的因素;采用Ka-plan-Meier 生存曲线分析miR-296-3p、miR-324-3p水平与肾癌患者预后的关系.结果:健康组、良性组、肾癌组血清miR-296-3p水平依次升高(P＜0.05),miR-324-3p水平依次降低(P＜0.05).miR-296-3p高表达患者TNM分期为Ⅲ～Ⅳ期的例数显著高于miR-296-3p低表达患者(P＜0.05);miR-324-3p低表达患者TNM分期为Ⅲ～Ⅳ期的例数显著高于miR-324-3p高表达患者(P＜0.05).生存组miR-296-3p水平显著低于死亡组,miR-324-3p水平显著高于死亡组(P＜0.05).TNM分期、miR-296-3p、miR-324-3p是影响肾癌患者生存或死亡的因素(P＜0.05).miR-296-3p高表达患者3年总生存率(43.75％,21/48)显著低于miR-296-3p低表达患者(95.24％,40/42)(x2=28.471,P＜0.001);miR-324-3p 低表达患者 3 年总生存率(43.48％,20/46)显著低于 miR-324-3p 高表达患者(93.18％,41/44)(x2=27.768,P＜0.001).结论:肾癌患者血清 miR-296-3p 水平显著升高,miR-324-3p水平显著降低,且与肾癌患者临床病理特征以及预后相关.
Abstract
Objective:To analyze the relationship between the expression levels of serum microribonucleic acid-296-3p(miR-296-3p),microribonucleic acid-324-3p(miR-324-3p)and the clinical pathological features,prognosis of renal cell carcinoma patients.Methods:A total of 90 renal cancer patients(renal cancer group)admitted to Guang'an People's Hospital from January 2017 to December 2020 were selected.Additionally,90 patients with be-nign renal lesions(benign group)admitted to our hospital were selected,and 90 healthy individuals(healthy group)who underwent physical examinations at our hospital during the same period were collected.Real-time fluores-cence quantitative PCR(RT-PCR)method was applied to detect the relative expression levels of miR-296-3p and miR-324-3p in serum.Cox regression was applied to analyze the factors affecting the prognosis of renal cancer pa-tients.Kaplan-Meier survival curve was applied to analyze the relationship between miR-296-3p,miR-324-3p lev-els and the prognosis of renal cancer patients.Results:The serum miR-296-3p level in the healthy group,benign group,and renal cancer group increased sequentially(P＜0.05),while the miR-324-3p level decreased sequentially(P＜0.05).The number of TNM stage Ⅲ-Ⅳ in patients with high expression of miR-296-3p was obviously higher than those in patients with low expression of miR-296-3p(P＜0.05).The number of TNM stage Ⅲ-Ⅳ in patients with low expression of miR-324-3p was obviously higher than that in patients with high expression of miR-324-3p(P＜0.05).The miR-296-3p level in the survival group was obviously lower than that in the death group,while the miR-324-3p level was obviously higher than that in the death group(P＜0.05).TNM staging,miR-296-3p,and miR-324-3p were factors that affected the survival or death of renal cancer patients(P＜0.05).The 3-year overall survival rate(43.75％,21/48)of patients with high expression of miR-296-3p was obviously lower than that of patients with low expression of miR-296-3p(95.24％,40/42)(x2=28.471,P＜0.001).The 3-year overall survival rate of patients with low expression of miR-324-3p(43.48％,20/46)was obviously lower than that of patients with high expression of miR-324-3p(93.18％,41/44)(x2=27.768,P＜0.001).Conclusion:High level of serum miR-296-3p and low level of serum miR-324-3p are associated with the clinical pathological features and prognosis of renal cancer patients.
关键词
肾癌/微小核糖核酸-296-3p/微小核糖核酸-324-3p/临床病理特征/预后Key words
renal cancer/microribonucleic acid-296-3p/microribonucleic acid-324-3p/clinical pathological features/prognosis引用本文复制引用
基金项目
四川省医学青年创新科研课题(Q22051)
四川省医疗卫生与健康促进会科研项目(KY2022QN0139)
出版年
2024
NETL
NSTL
万方数据