Relationship between serum miR-296-3p and miR-324-3p expression and clinical pathological features and prognosis of renal cell carcinoma patients
Objective:To analyze the relationship between the expression levels of serum microribonucleic acid-296-3p(miR-296-3p),microribonucleic acid-324-3p(miR-324-3p)and the clinical pathological features,prognosis of renal cell carcinoma patients.Methods:A total of 90 renal cancer patients(renal cancer group)admitted to Guang'an People's Hospital from January 2017 to December 2020 were selected.Additionally,90 patients with be-nign renal lesions(benign group)admitted to our hospital were selected,and 90 healthy individuals(healthy group)who underwent physical examinations at our hospital during the same period were collected.Real-time fluores-cence quantitative PCR(RT-PCR)method was applied to detect the relative expression levels of miR-296-3p and miR-324-3p in serum.Cox regression was applied to analyze the factors affecting the prognosis of renal cancer pa-tients.Kaplan-Meier survival curve was applied to analyze the relationship between miR-296-3p,miR-324-3p lev-els and the prognosis of renal cancer patients.Results:The serum miR-296-3p level in the healthy group,benign group,and renal cancer group increased sequentially(P＜0.05),while the miR-324-3p level decreased sequentially(P＜0.05).The number of TNM stage Ⅲ-Ⅳ in patients with high expression of miR-296-3p was obviously higher than those in patients with low expression of miR-296-3p(P＜0.05).The number of TNM stage Ⅲ-Ⅳ in patients with low expression of miR-324-3p was obviously higher than that in patients with high expression of miR-324-3p(P＜0.05).The miR-296-3p level in the survival group was obviously lower than that in the death group,while the miR-324-3p level was obviously higher than that in the death group(P＜0.05).TNM staging,miR-296-3p,and miR-324-3p were factors that affected the survival or death of renal cancer patients(P＜0.05).The 3-year overall survival rate(43.75％,21/48)of patients with high expression of miR-296-3p was obviously lower than that of patients with low expression of miR-296-3p(95.24％,40/42)(x2=28.471,P＜0.001).The 3-year overall survival rate of patients with low expression of miR-324-3p(43.48％,20/46)was obviously lower than that of patients with high expression of miR-324-3p(93.18％,41/44)(x2=27.768,P＜0.001).Conclusion:High level of serum miR-296-3p and low level of serum miR-324-3p are associated with the clinical pathological features and prognosis of renal cancer patients.
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