Changes of glycocalyx fractions in different types of anti-neutrophil cytoplasmic antibody-associated vasculitis and their correlation with disease activity
Changes of glycocalyx fractions in different types of anti-neutrophil cytoplasmic antibody-associated vasculitis and their correlation with disease activity
Objective To explore changes of glycocalyx fractions in different types of anti-neutrophil cytoplasmic antibody-associated(ANCA)vasculitis(AAV)and their correlation with disease activity.Methods A total of 49 AAV patients(AAV group)and 53 healthy persons(control group)were enrolled.Six monosaccharides levels in serum after polysaccharide degradation were detected by PCR-assisted acid degradation and high performance liquid chromatography in subjects of above two groups.Serum degradation monosaccharide levels between AAV group and control group,between granulomatous polyvasculitis(GPA)and microscopic polyvasculitis(MPA)patients,between peroxidase(MPO)-ANCA and protease 3(PR3)-ANCA positive AAV patients,among inactive,active and highly active AAV patients were compared.Spearman correlation analysis was used to assess correlation between serum degraded monosaccharide levels and clinical index in AAV patients.Receiver operating characteristic(ROC)curve was used to analyze predictive value of serum degraded galactosamine(GalN_de)level for disease high activity in MPO-ANCA positive AAV.Results Serum degraded fucose(Fuc_de),GalN_de,degraded glucose(Glc_de),degraded mannose(Man_de)and total serum degraded monosaccharides levels in AAV group were all higher than those in control group(P＜0.05).Total serum degraded monosaccharides level in MPA patients was higher than that in GPA patients(P＜0.05).Total serum degraded monosaccharides level in MPO-ANCA positive AAV patients was higher than that in PR3-ANCA positive AAV patients(P＜0.05).Serum GalN_de level was different significantly among inactive,active and highly active AAV patients,and it in highly active patients was higher than that in inactive patients(P＜0.05).Spearman correlation analysis showed that serum fuc_de was negatively correlated with blood neutrophil count and erythrocyte sedimentation rate(ESR)in AAV patients,glc_de,man_de and total serum degraded monosaccharides were positively correlated with ESR and c-reactive protein(CRP)(P＜0.05).ROC curve analysis showed that area under ROC curve(AUC)of serum galn_de level for predicting disease high activity in MPO-ANCA positive AAV was 0.838(95％CI 0.695～0.982,P=0.009),and optimal cut-off value was 0.204 mmol/L,sensitivity was 83.3％and specificity was 79.4％.Conclusion Monosaccharide levels of serum degraded glycocalyx are correlated with disease type and disease activity in AAV patients.
维普
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