Objective To investigate the effect and mechanism of tumor suppressor gene 7L(ST7L)on the proliferation and apoptosis of pancreatic cancer cells.Methods Plasma samples of 22 pancreatic cancer patients and 22 healthy controls plasma samples were collected.13 pairs of pancreatic cancer tissue and corresponding paracancer tissue specimens were collected.Pancreatic cancer cells were transfected with ST7L overexpression plasmid and blank plasmid respectively as ST7L group and control group,ST7L knockdown plasmid and blank plasmid respectively as si-ST7L group and si-NC group.The quantitative reverse transcription(qRT)-PCR was performed to analyze ST7L expression in clinical samples.Western blotting were used to detect the expression level of ST7L in pancreatic cancer cells.Cell counting kit-8(CCK-8)method was used to detect cell proliferation,flow cytometry was used to detect cell apoptosis,western blot was used to detect the expression levels of related proteins and compared in groups.Results The expression level of ST7L in plasma of pancreatic cancer patients was significantly lower than that of healthy controls,and the expression level of ST7L in pancreatic cancer tissues was significantly lower than that in adjacent tissues;the expression level of ST7L in pancreatic cancer cell lines PANC-1,AsPC-1 and PaCa-2 was significantly lower than that in normal pancreatic ductal cell line H6C7(P＜0.05).Proliferation ability of ST7L group and control group increased in turn at 24 h,48 h,72 h and 96 h.Proliferation ability of ST7L group at 24 h,48 h,72 h and 96 h was significantly lower than that in control group at the same period(P＜0.05).Apoptosis rates of PANC-1 and PaCa-2 in ST7L group were higher than those in control group;expression of apoptosis-related protein Caspase-3 in PANC-1 and PaCa-2 cells in ST7L group was higher than that in control group,while expression of Bcl-2 was lower than that in control group(respectively,P＜0.05).The expressions of β-catenin,c-Myc and Cyclin D1 in ST7L group were lower than those in control group.Expressions of β-catenin,c-Myc and Cyclin D1 in si-ST7L group were higher than those in si-NC group(P＜0.05).Conclusion ST7L may inhibit the growth of pancreatic cancer cells by reducing the expression of Wnt/β-catenin signaling pathway related proteins.
维普
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