Tce-MEP对大鼠坐骨神经显微吻合术后的评估价值
Evaluation value of Tce-MEP in rat sciatic nerve after microsurgical anastomosis
邵晓雨 1宋启民 2车峰远3
作者信息
- 1. 264003,山东烟台,滨州医学院第二临床医学院
- 2. 临沂市人民医院神经外科
- 3. 临沂市神经生理学重点实验室
- 折叠
摘要
目的 探讨经颅电刺激运动诱发电位(Tce-MEP)对大鼠坐骨神经断裂损伤动物模型显微外科手术吻合术后的评估价值.方法 将18只大鼠随机分为对照组(Sham组)、坐骨神经断裂损伤吻合手术组(Model组)、坐骨神经断裂损伤不吻合组(SNI组),每组各6只.Sham组只行坐骨神经分离术不损伤坐骨神经,SNI组和Model组大鼠在距梨状肌下缘1 cm处切断坐骨神经干,Model组立即行神经外膜端端吻合,SNI组不吻合.各组均记录大鼠术前及术后第2天、第7天、第14天和第30天的Tce-MEP潜伏期及波幅;分别在术前及术后第2天、第7天、第14天和第30天行大鼠Bs-so-Beattie-Bresnahan(BBB)运动功能评分;各组均在术后第30天取材,采用苏木精-伊红(HE)染色观察大鼠坐骨神经的组织形态学改变,采用透射电镜观察其坐骨神经组织超微结构变化.结果 Mod-el 组和SNI组所有大鼠坐骨神经剪断后均出现Tce-MEP波形变化,Model组吻合术前表现为潜伏期延长及波幅降低(P＜0.05),术后不同时间段复测波形均有所恢复.Model组和SNI组术后各时间点均较术前BBB评分降低(P＜0.05)o HE染色法和透射电镜显示,Model组坐骨神经远端脱髓鞘现象较SNI组好转.结论 显微外科吻合手术可以促进大鼠坐骨神经断裂损伤的神经功能修复,且可以减轻神经损伤远端的脱髓鞘改变;Tce-MEP可以连续客观地评估大鼠坐骨神经断裂损伤修复后不同时间点的神经传导功能恢复情况.
Abstract
Objective To explore the evaluation value of transcranial electrical motor evoked po-tential(Tce-MEP)in rat sciatic nerve rupture injury after microsurgical anastomosis.Methods A to-tal of 18 rats were randomly divided into a control group(Sham group),model group(a sciatic nerve fracture injury+anastomosis)and SNI group(a sciatic nerve fracture injury),with 6 rats in each group.Sham group underwent sciatic nerve dissection without damaging the sciatic nerve,while the sciatic nerve trunks in SNI and Model groups were cut at a distance of 1 cm from the low edge of the piriformis muscle.Model group was given end to end anastomosis of the outer nerve membrane,while SNI group was not given anastomosis.Tce-MEP latency and wave amplitude of the rats were recorded before surgery and on days 2nd,7th,14th and 30th after surgery.Bsso Beattie Bresnahan(BBB)was perform to score rat motor function before surgery and on the days 2nd,7th,14th and 30th after surger-y.Samples were taken from each group on the 30th day after the surgery,and hematoxylin-eosin(HE)staining was conducted to observe the morphological changes of rat sciatic nerve tissues.Ultrastruc-tural changes of rat sciatic nerve tissues were observed by transmission electron microscopy.Results All rats in model and SNI groups had Tce-MEP waveform changes after cutting sciatic nerves.Model group showed prolonged latency and decreased amplitude before anastomosis(P＜0.05),and re-ex-aminations at different periods showed that the waveforms were recovered after surgery.BBB scores of Model and SNI groups at each time points after surgery were lower than those of before surgery(P＜0.05).HE staining and transmission electron microscopy showed that the demyelination of the distal sciatic nerves was improved in Model group when compared to SNI group.Conclusion Microsurgical anastomosis may promote the repair of nerve function and reduce demyelination at the distal end of sci-atic nerve injury.Tce-MEP can be used to continuously and objectively evaluate the recovery of nerve conduction function at different times after the repair of sciatic nerve injury.
关键词
经颅电刺激运动诱发电位/坐骨神经损伤/动物模型/端端吻合/神经传导功能Key words
Tce-MEP/sciatic nerve injury/animal model/end-to-end anastomosis/nerve con-duction function引用本文复制引用
基金项目
山东省医药卫生科技面上项目(202304040611)
出版年
2024
NETL
NSTL
万方数据