Objective To investigate the effects of anlotinib on the adhesion,migration,invasion and epithelial-mesenchymal transition(EMT)of human glioma cells.Methods Human glioma T98G cells were cultured in vitro and divided into control group(no intervention),experimental group(2.5 μmol·L-1 anlotinib group,5 μmol·L-1 anlotinib group,10 μmol·L-1 anlotinib group)and positive drug group(50 mg·L-1 5-fluorouracil)for 24 hours.Cell proliferation,adhesion,migration,invasion and EMT-related protein expression were analyzed by cell count(CCK-8),cell adhesion assay,scratch assay,Transwell and Western blotting(WB).Results Compared with the control group,the proliferative activity of 5 μmol·L-1,10 μmol·L-1 and positive drug groups was significantly decreased(P＜0.05),and the adhesion,migration and invasion ability of T98G cells in the experimental group and positive drug group were significantly decreased(P＜0.05).The expression levels of fibrin(FN),Vimentin and N-cadherin were significantly down-regulated(P＜0.05),while the expression levels of E-cadherin were significantly up-regulated(P＜0.05).The higher the concentration,the more significant the change.Compared with positive drug group,the cell proliferation activity,adhesion,migration invasion ability,and the protein levels of N-cadherin,Vimentin and FN were up-regulated,while the protein level of E-cadherin was down-regulated in the 2.5 μmol·L-1 and 5 μmol·L-1 anlotinib groupsd(P＜0.05).There was no significant difference between the 10 μmol·L-1 antirotinib group and the positive group(P＞0.05).Conclusion Anlotinib may inhibit the proliferation,adhesion,migration and invasion ability of human glioma T98G cells by inhibiting the process of EMT.
维普
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