Objective To investigate the clinical manifestations,diagnosis,treatment and prognosis of intraspinal hematoma(ISH)caused by lumbar puncture(LP).Methods The clinical data of 2 patients admitted to The First Affiliated Hospital of Xinxiang Medical College from July 2020 to October 2020 with acute ISH caused by LP in the presence of antiplatelet and anticoagulant drugs were analyzed retrospectively,and the relevant literature were reviewed.Results Both cases had ruptured cerebral aneurysms complicated with subarachnoid hemorrhage,in which the antiplatelet aggregation and anticoagulant drugs were used to prevent stent thrombosis after the stent-assisted coil embolization of aneurysms.Acute ISH occurred in the 2 patients after LP,and further hematoma removal and spinal decompression were performed.Both cases were discharged with varying degrees of neurological dysfunction.Conclusions With the application of anticoagulant and antiplatelet aggregation drugs,LP is more likely to lead to the formation of ISH.The diagnosis mainly depends on the clinical manifestations,signs and imaging examination of the patients.The treatment is mainly surgical decompression,and the prognosis is mainly related to the degree of neurological function loss at the onset.