Impacts of sevoflurane on malignant biological behavior of gastric cancer cells by regulating miR-96-5p/SIK1 axis
Objective The objective of this study was to investigate the impacts of sevoflurane on the proliferation,invasion and apoptosis of gastric cancer(GC)cells by regulating the expression of microRNA(miR)-96-5p/salt induced kinase 1(SIK1)axis.Methods GC cell SGC7901 cells were treated with sevoflurane of 0％,1％,2％,3％,4％,5％v/v concentration,the proliferation of SGC7901 cells was detected by CCK-8 method;SGC7901 cells were randomly grouped into three groups:control group(Normal culture),sevoflurane group(3％v/v sevoflurane intervention),sevoflurane+miR-NC group(Transfected with miR-NC and then treated with sevoflurane at 3％v/v concentration for 48 h),sevoflurane+miR-96-5p mimics group(After transfection with miR-96-5p mimics,intervention was performed at 3％v/v concentration of sevoflurane for 48 h),RT-qPCR was applied to detect the expression of miR-96-5p in cells of each group;flow cytometry was applied to detect apoptosis;scratch test was applied to detect cell migration;Transwell cell test was applied to detect cell invasion;Western blot was applied to detect the expression of phosphorylate-induced kinase 1(SIK1),matrix metalloproteinase-2(MMP-2)and MMP-9;double luciferase reporter gene experiment was applied to verify the targeting relationship between miR-96-5p and SIK1.Results Compared with the 0％concentration group,the inhibition rate of SGC7901 cell proliferation was obviously increased in a dose-dependent manner under the treatment of 1％,2％,3％,4％,and 5％v/v concentration of sevoflurane(P＜0.05),the 3％v/v concentration of sevoflurane was selected for subsequent experiments;compared with the control group,the expression level of miR-96-5p,migration,invasion,and the expression levels of MMP-2 and MMP-9 proteins in sevoflurane group were obviously decreased,the apoptosis rate and the expression level of SIK1 protein were obviously increased(P＜0.05);activation of miR-96-5p expression weakened the inhibition of sevoflurane on the migration and invasion of SGC7901 cells,and decreased the apoptosis rate(P＜0.05);the results of double luciferase reporter gene showed that miR-96-5p could target the expression of SIK1.Conclusion Sevoflurane may inhibit the proliferation,migration and invasion of GC SGC7901 cells and promote cell apoptosis by down-regulating miR-96-5p and up-regulating SIK1.
sevofluranemicroRNA-96-5p/salt induced kinase 1 axisgastric cancer cellsproliferationapoptosis
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维普
