[Objective]To quantitatively detect the expression of serum micro-RNA-497(miR-497)in pa-tients with pancreatic cancer(PC)and to explore its clinical significance.[Methods]Real-time fluorescence quantitative RT-PCR was used to detect the expression of miR-497 in serum of 70 patients with PC before treatment and 30 healthy controls,and the levels of CA199 and CEA in serum were detected at the same time.Patients with PC were followed up for 2 years after treatment.Clinical value of the expression of ser-um miR-497 was analyzed.[Results]The expression of serum miR-497 in PC group before treatment was significantly lower than that in normal control group(P＜0.01).The sensitivity of serum miR-497 in the diagnosis of pancreatic cancer was significantly higher than that of serum CA199(91.0％vs 76.6％,x2=7.637,P＜0.01).There was no significant difference in diagnostic accuracy between the two groups(P＞0.05).The combined detection of the three items could improve the sensitivity and specificity of diagno-sis.The expression of serum miR-497 before treatment was negatively correlated with the depth of PC inva-sion,lymph node metastasis and distant metastasis(P＜0.01).The 1-year and 2-year survival rate in the group with low expression of serum miR-497 before treatment was significantly lower than that in the group with high expression after treatment(P＜0.05).Cox multivariate analysis showed that the expres-sion of serum miR-497 before treatment was an independent high risk factor affecting the prognosis of PC.[Conclusion]The low expression of serum miR-497 is closely related to the invasion,metastasis and poor prognosis of PC.It has a certain value in the diagnosis of PC,and can be used as an index for clinical auxil-iary diagnosis and prognosis evaluation of PC.
关键词
胰腺癌/microRNA-497/实时荧光定量RT-PCR/侵袭转移/预后
Key words
pancreatic cancer/mic roRNA-497/real time fluorescence quantitative RT-PCR/invasion and metastasis/prognosis
维普
万方数据