The application of SEPT9 gene methylation levels in the differential diagnosis of bladder urinary tract epithelial lesions
Purpose This study aimed to explore the diag-nostic role of SEPT9 gene methylation levels in distinguishing between benign and malignant urinary epithelial lesions and to investigate the potential of reducing the false negative rate of bladder biopsy tissue by combining SEPT9 methylation testing.Methods SEPT9 methylation was used to detect 160 cased of pathologically diagnosed urinary tract lesions,and the sensitivity and specificity of its auxiliary diagnosis of benign and malignant lesions were analyzed.The methylation levels of the SEPT9 gene were compared between cancerous and para-cancerous tissues.SEPT9 gene methylation levels were also assessed in pathological tissues initially misdiagnosed as inflammation or benign lesions on bladder biopsy,but later confirmed as cancer through re-bi-opsy or resection.Results The sensitivity and specificity of SEPT9 gene methylation levels in distinguishing between benign and malignant urinary tract epithelial lesions were 98％(88/90)and 94％(66/70),respectively.Among non-cancerous adjacent tissues,47％(7/15)showed positive SEPT9 gene methylation,indicating aberrant epigenetic modifications in these tissues.Among 12 cases initially diagnosed as inflammation or benign lesions on cystoscopic biopsy,the positive rate of SEPT9 gene methylation was 67％(8/12).After repeat biopsy or path-ological resection,SEPT9 methylation detection in tissues con-firmed as cancer was 100％positive(12/12).Conclusion SEPT9 gene methylation detection can aid in the differentiation of benign and malignant urothelial lesions,which potentially re-ducing the false negative rate of bladder biopsy tissue.
万方数据
维普
