Analysis HER2 expression and clinicopathologic analysis in urothelial carcinoma of the bladder
Purpose To investigate the protein expression of human epidermal growth factor receptor 2(HER2)in bladder urothelial carcinoma(BUC)tissues and to analyze its clinical significance.Methods 27 cases of non-invasive low-grade BUC,5 cases of non-invasive high-grade BUC and 60 cases of invasive high-grade BUC of the bladder were collected.The im-munohistochemical method(IHC)detection technique was used to detect the expression of HER2 protein in each tissue sample and analyze the relationship between the expression of HER2 and clinicopathological characteristics of BUC.The relationship be-tween HER2(ERBB2)expression and BUC was analyzed indi-rectly through the GEPIA database or directly in the TCGA(The Cancer Genome Atlas).Results The HER2 2+/3+expres-sion rate of invasive high-grade BUC(26.67％,16/60)was significantly higher than non-invasive low-grade BUC(7.41％,2/27)and non-invasive high-grade BUC(0,0/5)(P＜0.05).The 2+/3+expression rate of HER2 of multifocal in-vasive high-grade BUC was 75.00％(6/8),which was signifi-cantly higher than that of unifocal invasive high-grade BUC,which was 19.23％(10/52)(P＜0.05).There was a signifi-cant difference in the expression of HER2 in the histological sub-types(P＜0.05),with the 2+/3+positive rate of the usual type being 30％(6/20),with adenoid differentiation type was 57.14％(4/7),micropapillary type was 66.67％(4/6),plasma cell-like type was 100.00％(2/2),and the rest of the subtypes were negatively expressed.There was no statistically significant relationship between HER2 expression and other clin-icopathologic features(P＞0.05).In the TGGA data,HER2(ERBB2)expression in BUC tumor tissues was higher than that in normal tissues(P＞0.05),and the overall survival(OS)of patients with high HER2(ERBB2)expression was lower than that of patients with low HER2(ERBB2)expression(P＞0.05);the results of the Cox univariate analysis showed that OS was shorter in patients with higher age,Stage,T and N.The a-bove significantly different factors were included in Cox multifac-torial analysis,which showed that age,Stage,T and N were in-dependent prognostic factors for BUC.Conclusion The 2+/3+expression rate of HER2 in invasive high-grade UBC was higher than that in non-invasive low-grade and non-invasive high-grade BUC and was closely associated with the number of tumor and histological subtypes.The HER2 expression status combined with the number of tumor and histological subtypes may be helpful in screening patients for potential HER2-targeted therapies effective in invasive high-grade BUC.
bladder urothelial carcinomainvasive high-grade uroepithelial carcinoma of the bladderHER2clinicopathologyimmunohistochemistry
万方数据
维普
