CD66b+tumor-associated neutrophils and desmoplastic reaction in colorectal carcinoma and relationship with clinicopathologic features and prognosis
Purpose To analyze the classification of CD66b+tumor-associated neutrophils(TANs)and desmoplastic reaction(DR)in colorectal cancer(CRC),and to explore the clinicopathological significance and prognostic value of CD66b+TANs and DR in CRC.Methods The expression of CD66b+TANs in 240 CRCs was detected by immunohistochemistry(IHC),and the classification of tumor stromal DR was observed by HE stained sections,and the relationship between CD66b+TANs and DR classification and clinicopathological features was studied.Kaplan-Meier survival curve was used to analyze the re-lationship between CD66b+TANs and DR and CRC prognosis.Results Among the 240 cases of CRC,29 cases were CD66b+TANs rich(NR)group and 211 cases were CD66b+TANs intermediate(NI)/deficiency(NP)group.In the DR classification,there were 92 cases of mature type,63 cases of intermediate type,and 85 cases of immature type.The CD66b+TANs NR group was associated with higher histological grade(P=0.034),later TNM stage(P=0.006),and vascular in-vasion(P=0.002).The classification of DR in CRC was close-ly related to the degree of tumor differentiation,nerve invasion,lymph node metastasis,and pTNM stage,while DR immature type CRC had poorly differentiation,neural invasion,lymph node metastasis,and later pTNM stage(P＜0.05).Kaplan-Meier survival analysis showed that the 3-year overall survival(OS)was significantly reduced(P＜0.05)in patients with CD66b+TANs NI/NP CRCs compared with CD66b+TANs NI/NP CRC patients.By multivariate analysis,high TANs was an independent prognostic factor(P＜0.05).The 3-year overall survival rate of patients with DR-immature CRC was significantly lower than that of patients with intermediate and mature CRC(P＜0.05).Conclusion CD66b+TANs and the degree of stro-mal DR in tumors can affect the clinical and biological behavior of CRC,and evaluating of CD66b+TANs and DR in CRC can help predict the prognosis of CRC patients.
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