Expression of EpCAM and HDAC6 in prostate cancer and their effects on cell migration
Purpose To observe the expression of epitheli-al cell adhesion molecule(EpCAM)and histone deacetylase(HDAC6)proteins in prostate cancer and benign tissue adjacent to cancer,to examine the relationship between the expression of EpCAM and HDAC6 proteins and clinicopathological character-istics and to investigate the impact of EpCAM silencing on pros-tate cancer cell migration and epithelia-mesenchymal transition(EMT).Methods The expression levels of EpCAM and HDAC6 mRNAs in different types of cancers were analyzed using TIMER and UALCAN database retrieval.The expression levels of EpCAM and HDAC6 proteins were observed in prostate cancer and benign tissue adjacent to cancer through immunohistochemi-cal staining.The correlation between these two proteins was also analyzed.The impact of silencing EpCAM protein on the migra-tion ability of prostate cancer cells was verified through cell scratch and migration experiments.The influence of EpCAM on EMT in prostate cancer cells was confirmed using Western blot analysis.Results The database analysis revealed that the ex-pression of EpCAM and HDAC6 mRNAs was higher in prostate cancer compared to normal prostate tissue.This expression was found to be associated with the Gleason score and lymph node metastasis.Immunohistochemical staining results demonstrated that the positive expression rate of EpCAM protein in prostate cancer tissue was 62.61％,which was significantly higher than that in benign tissue adjacent to cancer(21.62％,P＜0.001).The high expression of EpCAM protein in prostate cancer was correlated with tumor size,infiltration,Gleason score,and WHO/ISUP grade(all P＜0.05),but not with age,PSA,and lymph node metastasis.Similarly,the positive expression rate of HDAC6 protein in prostate cancer tissue was 81.74％,which was significantly higher than that in benign tissue adjacent to cancer(43.24％,P＜0.001).However,there was no signifi-cant correlation with age,tumor size,infiltration,Gleason score,PSA,and lymph node metastasis.The starbase data re-trieval results indicated a positive correlation between EpCAM and HDAC6 mRNAs expression in prostate cancer tissues(r=0.206,P=3.44e-06).A positive correlation between EpCAM and HDAC6 proteins expression in prostate cancer tissue was ob-served through the Spearman rank correlation test(r=0.454,P＜0.001).Following si-EpCAM transfection into DU-145 prostate cancer cells,the expression of EpCAM and HDAC6 proteins decreased.The si-EpCAM group exhibited a significant downregulation in migration and invasion ability compared to the si-NC group(P＜0.001).Additionally,the expression of the EMT related protein E-cadherin was upregulated,while the ex-pression of vimentin was downregulated.Conclusion The high-er expression level of EpCAM and HDAC6 proteins in prostate cancer tissues suggests a relationship with the occurrence,devel-opment,and degree of malignancy.Silencing the expression of EpCAM protein in prostate cancer cells also reduces the expres-sion of HDAC6 protein,inhibits DU 145 cell migration and rever-ses EMT progression.EpCAM and HDAC6 have the potential to serve as markers for diagnosis and prognosis of prostate cancer.
万方数据
维普
