Expression and clinical significance of CH25H and STAT3 in follicular lymphoma
Purpose To investigate the expression and sig-nificance of CH25H and STAT3 in follicular lymphoma(FL).Methods Differentially expressed genes in FL and normal lymph node tissues from the GEO were screened.Paraffin sam-ples from 54 cases of FL and 20 cases of reactive proliferative lymph node tissue were collected.Immunohistochemical methods were used to detect the expression of CH25H and STAT3 in par-affin samples,and their relationship with the clinical and patho-logical characteristics of FL was analyzed.Results The expres-sion of CH25H mRNA in FL was significantly higher than that in normal lymph node tissue(P＜0.01).The expression level of CH25H mRNA in FL was linearly correlated with STAT3(P＜0.01).The high expression rate of CH25H in FL was 48.1％,which significantly higher than that in reactive proliferative lymph node tissue(15.0％,P＜0.05).The high expression rate of STAT3 was 59.3％,which significantly higher than that of reactive proliferative lymph node tissue(25.0％,P＜0.05).The expression level of CH25H protein was significantly correla-ted with the pathological grading and clinical Ann Arbor staging of FL(all P＜0.05).CH25H expression was higher in FL with high Ki67 expression(≥30％),while it was lower in FL with low Ki67 expression,and the difference was significant(P＜0.05).There was no significant correlation between CH25H and patient age,gender,presence or absence of B symptoms,ex-pression of CD10,BCL6,BCL2,FLIPI-1 score,lymphocyte count,and monocyte count(all P＞0.05).The expression lev-el of STAT3 protein was significantly correlated with the patho-logical grading,clinical Ann Arbor staging,and FLIPI-1 score of FL(all P＜0.05).STAT3 expression was higher in FL with high Ki67 expression(≥30％),while it was lower in FL with low Ki67 expression,and the difference was significant(P＜0.05).STAT3 was not significantly correlated with patient age,gender,presence or absence of B symptoms,expression of CD10,BCL6,BCL2,lymphocyte count,and monocyte count(all P＞0.05).The expression levels of CH25H and STAT3 in FL were positively correlated(r=0.573,P＜0.001).Conclu-sion The expression levels of CH25H and STAT3 proteins in FL are significantly increased,which can serve as potential mo-lecular markers for pathological diagnosis,grading,and progno-sis evaluation of FL.Abnormal expression of CH25H and STAT3 may jointly participate in the occurrence and progression of FL.
万方数据
维普
