Clinical study of CTRP9 improving early cardiac function after myocardial infarction by regulating M1/M2 macrophage polarization in rats
Objective To investigate the mechanism of C1q-tumor necrosis factor-related protein-9(CTRP9)improving early cardiac function after myocardial infarction by regulating M1/M2 macrophage polarization in rats.Methods Sixty SD rats were randomly divided into control group,model group and intervention group,with 20 rats in each group.Rats in the model group and the intervention group were made into myocardial infarction model by ligating the left anterior descending coronary artery,and the intervention group received pcDNA3.1-CTRP9 recombinant plasmid intervention.Then various indexes were compared among groups,including general condition,myocardial infarction area,cardiac function[left ventricular ejection fraction(LVEF),left ventricular end diastolic diameter(LVDd),left ventricular end-systolic dimension(LVDs),functional shortaxial shortening rate(FS)],cardiomyocyte apoptosis rate,M1/M2 macrophage polarization[M1 type macrophages F4/80 positive and inducible nitric oxide synthase(iNOS)positive percentage,M2 type macrophages F4/80 positive and arginase 1(ARG-1)positive percentage],and protein expression of CTRP9 in myocardial tissue.Results The mental state,hair growth,dietary activity,and survival of rats in intervention group were better than those in model group.The myocardial cell apoptosis rate,myocardial infarction area,LVDs,LVDd,M1 type macrophage F4/80 positive and iNOS positive percentage in model group and intervention group were higher than those in control group(P＜0.05).The myocardial cell apoptosis rate,myocardial infarction area,LVDs,LVDd and M1 type macrophages F4/80 positive and iNOS positive percentage in intervention group were significantly lower than those in model group(P＜0.05).The percentage of LVEF,FS and M2 type macrophages F4/80 positive and ARG-1 positive percentage in model group and intervention group was lower than those in control group(P＜0.05).The LVEF,FS and M2 type macrophages F4/80 positive and ARG-1 positive percentage in the intervention group was higher than that in the model group(P＜0.05).The expression of CTRP9 protein in myocardium of the model group was lower than that of the control group(P＜0.05),and the expression of CTRP9 protein in myocardium of the intervention group was higher than that of the model group and the control group(P＜0.05).Conclusions CTRP9 can improve the early cardiac function after myocardial infarction by promoting the polarization of M1/M2 macrophages.
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