Role of imbalance of lymphocyte subsets in the pathogenesis of lung cancer and its relationship with tumor differentiation and survival rate of patients
Objective To investigate the role of lymphocyte subsets imbalance in the pathogenesis of lung cancer and its relationship with tumor differentiation and survival rate.Methods Eighty-eight lung cancer patients admitted to Xuchang Central Hospital from January 2019 to January 2021 were selected as the lung cancer group,and another 80 health examinees from Xuchang Central Hospital at the same time were selected as the health group.The peripheral blood lymphocyte subsets of all subjects were detected by flow cytometry.The relationship between the levels of T lymphocyte subsets and different clinical stages,differentiation levels,and pathological types of lung cancer patients were analyzed.The patients in the lung cancer group were followed up for 1 year to compare the lymphocyte subsets of patients with different prognosis,and the ROC curve was used to analyze the value of lymphocyte subsets in predicting the survival rate of lung cancer patients after one year of chemotherapy.Results The levels of CD3+,CD4+,and NK cells in the lung cancer group were significantly lower than those in the healthy group,while the levels of CD8+were higher than those in the healthy group(P＜0.05).As the degree of differentiation in lung cancer patients increases,the levels of CD3+,CD4+,and NK cells in their peripheral blood show a significant upward trend,while CD8+shows the opposite trend(P＜0.05).There was significant difference in the proportion of peripheral blood lymphocyte subsets among the patients with different pathological types of lung cancer(P＜0.05).The proportion of CD3+,CD4+,and NK cells in the peripheral blood of patients with stageⅢ-Ⅳ group was significantly lower than those of patients with stage Ⅰ-Ⅱ group(P＜0.05),while the proportion of CD8+T cells was higher than that of patients with stage Ⅰ-Ⅱ,with significant differences(P＜0.05).The Spearman rank analysis results showed that the levels of CD3+,CD4+,and NK cells were negatively correlated with lung cancer stage and positively correlated with differentiation degree,while the level of CD8+cells were positively correlated with lung cancer stage and negatively correlated with differentiation degree(P＜0.05).After a one-year survival follow-up,31 patients out of 88 lung cancer patients died,with a mortality rate of 35.23％.The levels of CD3+,CD4+,and NK cells in the death group were significantly lower than those in the survival group,while the level of CD8+was significantly higher than that in the survival group(P＜0.05).The ROC curve analysis results showed that the ROC AUC of CD3+,CD4+,CD8+,and NK cell levels predicting 1-year survival rate of lung cancer patients after chemotherapy were 0.838(0.721～0.955),0.826(0.708～0.944),0.708(0.554～0.862),and 0.618(0.618～0.900),respectively,with truncation values of 65.38％,31.19％,25.21％,and 18.36％.Predicted value:CD3+＞CD4+＞CD8+＞NK.Conclusions The immune status of patients with lung cancer is weaker than that of healthy people,and the level of lymphocyte subsets is correlated with the clinical stage and differentiation degree of the tumor,which could involved in the development and occurrence of lung cancer,and has a good predictive effect on the prognosis of patients with lung cancer.
Lymphocyte subsetsLung cancerSteady imbalanceElderly patientsDegree of differentiationPathological featuresPrognostic evaluation
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