The expression and clinical significance of RNF135 in esophageal squamous cell carcinoma
Objective To study the expression and clinical significance of ring finger protein 135(RNF135)in esophageal squamous cell carcinoma(ESCC).Methods ESCC tissues and adjacent tissues of 90 ESCC patients from January 2015 to December 2016 were collected.Three data sets of ESCC(GSE118037,GSE132867,GSE116958)were obtained from Gene Expression Omnibus(GEO)database by bioinformatics method,and the online tool GEO2R screened differentially expressed genes.The most significant differentially upregulated gene RNF135 was screened.Western blotting was used to detect the expression of RNF135 in three groups of cancer tissues and adjacent tissues,and immunohistochemistry was used to detect the expression of RNF135 in different WHO grade of 90 ESCC tissues.The Kaplan-Meier method was used to analyze the total survival time(OS)of patients with different RNF135 expression levels.Results Ninety-three differentially expressed genes including 44 up-regulated and 49 down-regulated genes in ESCC were screened through the intersection of three data sets.RNF135,the most significantly up-regulated gene was chosen for the following experiments.Western blotting detection showed an increase in the expression of RNF135 in ESCC tissues compared to adjacent cancer tissues(P<0.01).Immunohistochemical examination showed that the high expression rate of RNF135 was 56.7%(51/90),while the low expression rate was 43.3%(39/90).The expression of RNF135 was related to WHO level(P<0.01),but not to gender,age,tumor location,maximum tumor diameter and surgical method(P>0.05).The median OS of the entire group was 20.4(95%CI:18.555-22.245)months.The median OS of the RNF135 low expression group was 27.6(95%CI:23.779-31.421)months,while the median OS of the RNF135 high expression group was 18.0(95%CI:16.270-19.730)months,with a statistically significant difference(P<0.01).Conclusion RNF135 expression is significantly increased in ESCC and is remarkably associated with poor prognosis of the patients.
Esophageal squamous cell carcinomaRing finger protein 135Gene analysisPrognosis
NETL
NSTL
万方数据
