Anagliptin inhibits CT-26 cell migration by regulating cytoskeletal protein production through SOD-1/ROS pathway
Objective To study the effect of dipeptidyl peptidase-4(DPP-4)inhibitor Anagliptin on colorectal cancer cell migration.Methods CT-26 cells were cultured in vitro.CCK-8 assay was used to detect cell viability.The expressions of superoxide dismutase-1(SOD-1)and superoxide dismutase-2(SOD-2)were detected by Western blotting.Cell migration ability was detected in Transwell chamber.Cytoskeleton protein(F-actin)and intracellular reactive oxygen species(ROS)were detected by immunofluorescence assay.Results CCK-8 assay showed that 2 mmol/L Anagliptin could promote apoptosis of CT-26 cells(P<0.01).After CT-26 cells were incubated with 1 mmol/L Anagliptin,the migration ability of CT-26 cells decreased significantly(0.375±0.028,P<0.01).Co-incubation of Anagliptin and Tempol(ROS scavger)could significantly inhibit the migration inhibition of CT-26 cells induced by Anagliptin(0.527±0.035,P<0.01)and intracellular ROS accumulation(1.395±0.055 3,P<0.01).After incubating Anagliptin in CT-26 cells,the expression of SOD-1 decreased(0.665±0.028,P<0.01),but the expression of SOD-2 did not change(P>0.05).The migration ability of CT-26 cells was significantly decreased after incubation with SOD inhibitor DDC(0.206±0.009,P<0.01).Co-incubation of Anagliptin and Tempol antagonized the decline in CT-26 cytoskeleton protein F-actin synthesis induced by Anagliptin.Conclusion Anagliptin inhibits CT-26 cell migration by regulating the production of cytoskeleton protein(F-actin)through SOD-1/ROS pathway.
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