Experimental study on expression of Tenascin-C in hepatocellular carcinoma and its effect on invasion and metastasis of hepatocellular carcinoma Hep3B and SMMC-7721 cells
Objective To investigate the expression of Tenascin-C in human hepatocellular carcinoma(HCC)and analyze the relationship between the expression level and invasion and metastasis of HCC cells.Methods Human immortable hepatocytes L-02,HL-7702 and human hepatocellular carcinoma cells Hep3B,HepG2,SMMC-7721,Bel-7402,HHCC,MHCC-97H,MHCC-97L were cultured in vitro.Real-time fluorescence quantitative PCR(qPCR)and Western blotting were used to detect the expression of Tenascin-C,and Transwell was used to detect the migration and invasion ability of human hepatoma cells.Tenascin-C small interfering RNA was constructed and Transwell was used to detect the changes of migration and invasion ability of hepatoma Hep3B and SMMC-7721 cells.The correlation between the expression of Tenascin-C and invasion and metastasis of hepatocellular carcinoma cells was analyzed.Results Compared with immortalized hepatocytes,the expression levels of Tenascin-C mRNA and protein in human hepatocellular carcinoma cells were increased,with statistical significance(P=0.001).The metastasis ability of human hepatocellular carcinoma cells is different,Hep3B is the highest,followed by SMMC-7721,Bel-7402,HepG2,MHCC97-L is the lowest.After Tenascin-C was silenced,hepatoma Hep3B and SMMC-7721 cells had decreased migration and invasion ability compared with the negative control group,and the difference was statistically significant(P=0.001).Correlation analysis showed that Tenascin-C was positively correlated with the number of liver cancer cell migration and invasion(P= 0.001).Conclusion The high expression of Tenascin-C in human hepatocarcinoma may be involved in promoting the invasion and metastasis of cancer cells.Silencing Tenascin-C can effectively inhibit the metastasis ability of hepatocarcinoma Hep3B and SMMC-7721 cells,which is expected to be an important biological indicator for the progression of hepatocarcinoma.
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