Targeted-regulation of TIMP2 expression by microRNA-483-5p and its effect on the proliferation and invasion of bladder cancer cells
Objective To investigate the impact of microRNA-483-5p(miR-483-5p)on the proliferation,migration,and invasion of bladder cancer(BC)cells as well as its potential molecular mechanism.Methods The expression of miR-483-5p in BC tissues and its relationship with prognosis were analyzed by TCGA database.Fluorescence-based real-time quantitative PCR was used to analyze the expression levels of miR-483-5p and tissue inhibitor of matrix metalloproteinases(TIMP)-2 in BC T24 cells.Transfection of miR-483-5p mimic and inhibitor was performed in T24 cells.The impact of miR-483-5p on cellular proliferation was evaluated through the implementation of a CCK-8 assay.Migration and invasion capabilities of T24 cells were assessed utilizing Transwell assays.The targeted interaction between miR-483-5p and TIMP-2 was demonstrated using a dual-luciferase reporter gene assay.Rescue experiments was performed to verify whether the biological functions of miR-483-5p play a role through TIMP-2.Results The expression of miR-483-5p in BC tissues was significantly higher compared to adjacent tissues(P＜0.05).Patients with high expression of miR-483-5p exhibited a lower overall survival rate than those with low expression(P＜0.05).The miR-483-5p mimic significantly enhanced the proliferation,migration,and invasion capacities of T24 cells(P＜0.05).The inhibition of miR-483-5p resulted in a significant decrease in proliferation,migration and invasion capacities of T24 cells(P＜0.05).The downstream targeted gene of miR-483-5p,TIMP2,was found to be involved in regulating proliferation,migration and invasion capacities of T24 cells(P＜0.05).Furthermore,the downregulation effects of miR-483-5p on these cellular processes can be reversed by using interfering TIMP2.Conclusion The expression of miR-483-5p is upregulated in BC and correlates with unfavorable prognosis in BC patients.Moreover,miR-483-5p exerts its oncogenic effects by suppressing the expression of TIMP2,thereby promoting proliferation,migration,and invasion of BC cells.
Bladder cancerMicroRNA-483-5pTissue inhibitor of matrix metalloproteinases-2ProliferationInvasion
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