Targeting regulation of Smad4 by microRNA-190b-5p and its effect on TGF-β1-induced migration,invasion and epithelial-mesenchymal transition of rectal cancer cells
Objective To explore the role of microRNA-190b-5p(miR-190b-5p)in the transforming growth factor β1(TGF-β1)-induced migration,invasion and epithelial-mesenchymal transformation(EMT)of rectal cancer cells.Methods Rectal cancer SW1463 cells were treated with miR-190b-5p mimic and/or TGF-β1.Real-time fluorescence quantitative PCR was used to measure the expression level of miR-190b-5p.The expressions of E-cadherin,Vimentin and SMAD family member 4(Smad4)were detected by Western blot.Transwell assay was used to detect the migration and invasion abilities of SW1463 cells.Dual luciferase reporter gene experiment was employed to verify the targeting relationship between miR-190b-5p and Smad4.Results Compared with CCD841 con cells,the expression level of miR-190b-5p in SW1463 cells was lower.After TGF-β1 induction,the expression level of miR-190b-5p in SW1463 cells was further decreased,which promoted the occurrence of EMT,enhanced cell migration and invasion,and increased the expression level of Smad4 protein.Overexpression of miR-190b-5p inhibited TGF-β1-induced EMT,weakened cell migration and invasion,and decreased the expression of Smad4 protein level.Conclusion MiR-190b-5p may block TGF-β/Smad signaling pathway-induced EMT,invasion and migration of rectal cancer cells by inhibiting Smad4 expression.MiR-190b-5p/Smad4 has the potential to become a therapeutic target for rectal cancer.
Rectal cancerMicroRNA-190b-5pTransforming growth factor β1SMAD family protein 4Epithelial-mesenchymal transition
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