Effects of GLTSCR2 on migration,invasion and p53 signaling pathway of ovarian cancer cells
Objective To investigate the biological role of glioma tumor suppressor candidate region gene 2(GLTSCR2)in proliferation,migration and invasion of ovarian cancer cells.Methods GEPIA database was conducted to analyze the GLTSCR2 expression in ovarian cancer tissues.Quantitative real-time PCR was applied to measure the GLTSCR2 expression in ovarian cancer cell lines(CAOV3,A2780,OVCAR3 and SKOV3).SKOV3 and OVCAR3 cells were tranfected with pcDNA3.1(empty plasmid)and pcDNA3.1-GLTSCR2(overexpression plasmid),and allocated into SKOV3-NC group and SKOV3-GLTSCR2 group(overexpression of GLTSCR2 in SKOV3 cells)or OVCAR3-NC group and OVCAR3-GLTSCR2 group(overexpression of GLTSCR2 in OVCAR3 cells).CCK-8,scratch and Transwell assays were performed to examine cell proliferation,migration and invasion.Western blot was used to detect the expressions of p53,p-p53-Ser15 and phosphatase and tensin homolog deleted on chromosome 10(PTEN).Results GLTSCR2 were under-expressed in ovarian cancer tissues and cell lines.The proliferative,migrative and invasive abilities of SKOV3 and OVCAR3 cells were inhibited in SKOV3-GLTSCR2 group and OVCAR3-GLTSCR2 group as compared with SKOV3-NC group and OVCAR3-NC group,respectively.The protein levels of p53,p-p53-Ser15 and PTEN in SKOV3-GLTSCR2 group and OVCAR3-GLTSCR2 were higher than those in SKOV3-NC group and OVCAR3-NC group,respectively.Conclusion GLTSCR2 inhibits the cell proliferation,migration,invasion,and activates p53 signaling pathway,and probably functions as a tumor suppressor gene in ovarian cancer.
Ovarian cancerGlioma tumor suppressor candidate region gene 2MigrationInvasionP53 signaling pathway
万方数据
维普
