Effects of LEF1-AS1 on migration and invasion of gastric cancer cells through Wnt/β-catenin signaling pathway
Objective To investigate the influence of lymphoid enhancer-binding factor 1 antisense RNA 1(LEF1-AS1)on the proliferation,migration and invasion processes of gastric cancer cells through Wnt/β-catenin signaling pathway.Methods Expression patterns of LEF1-AS1 in gastric cancer cell lines and human gastric epithelial cell GES-1 were accessed by real time-quantitative polymerase chain reaction(qPCR).BGC-823 cells were divided into Negative control(NC)group,LEF1-AS1 interference(si-LEF1-AS1)group and si-LEF1-AS1+Wnt activator group.The CCK-8 kit was applied to detect cell viability.The wound healing and Transwell assays were employed to test the migration and invasion abilities of cells.Moreover,qPCR and Western blot were applied to detect the expressions of Wnt1,β-catenin and Survivin.Results LEF1-AS1 were remarkably upregulated in gastric cancer cell lines compared to GES-1 cell(P＜0.05).Compared with NC group,the abilities of proliferation,migration and invasion of BGC-823 cells were declined in si-LEF1-AS1 group(P＜0.05).Expression levels of Wnt1 and β-catenin in si-LEF1-AS1 group were decreased as compared with NC group(P＜0.05).In addition,the abilities of proliferation,migration and invasion of BGC-823 cells in si-LEF1-AS1+Wnt activator group were enhanced as compared with si-LEF1-AS1 group(P＜0.05).Expression levels of β-catenin and Survivin in si-LEF1-AS1+Wnt activator group were increased as compared with si-LEF1-AS1group(P＜0.05).Conclusion LEF1-AS1 silencing negatively regulates the biological behavior of gastric cancer cells via inhibiting Wnt/β-catenin signaling pathway activation.It has potential as a therapeutic target for gastric cancer.
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