Effects of LINC00092 on migration and invasion of osteosarcoma cells by targeting microRNA-373-5p
Objective To investigate the effects of long intergenic non-protein coding RNA 92(LINC00092)on migration and invasion progression of osteosarcoma cells as well as the underlying mechanism.Methods Quantitative real-time PCR(qPCR)was performed to detect the expression of LINC00092 in human normal osteoblasts hFOB1.19 cell line and osteosarcoma cell lines(SOSP-9607,U2-OS,Saos-2 and MG-63).MG-63 cells were cultured and divided into LINC00092 group(overexpression of LINC00092),pcDNA group(negative control)and Control group(blank control).MTT,Transwell assay and scratch assay were performed to determine proliferation,invasion and migration abilities of MG-63 cells.Moreover,the targeting relationship between microRNA-373-5p(miR-373-5p)and LINC00092 was verified by dual-luciferase reporter assay,qPCR and Western blot.Results Compared with hFOB1.19 cells,LINC00092 was significantly under-regulated in osteosarcoma cells(P＜0.05).The cell vitality,healing rate of cell scratches and the number of cell penetrating membranes in LINC00092 group significantly decreased compared with pcDNA group.MiR-373-5p was able to act as a downstream target of LINC00092,and negatively regulated by LINC00092(P＜0.05).Additionally,the expression of proliferating cell nuclear antigen(PCNA)and matrix metallopeptidase 9(MMP9)were inhibited in LINC00092 group on both mRNA and protein levels(P＜0.05).Conclusion LINC00092 targets miR-373-5p,regulates PCNA and MMP9 expression,and suppresses the osteosarcoma cells'proliferation,migration and invasion.
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