Effects of long non-coding RNA NUTM2A-AS1 on proliferation,migration and invasion of non-small cell lung cancer cells by regulating MAPK signaling pathway
Objective To determine the biological function of NUTM2A antisense RNA 1(NUTM2A-AS1)in regulating non-small cell lung cancer(NSCLC)cells'proliferation,migration and invasion through mitogen-activated protein kinase(MAPK)signaling pathway.Methods Expression of NUTM2A-AS1 in normal human lung epithelial cells BEAS-2B and human NSCLC cells were determined by real time-quantitative polymerase chain reaction.A549 cells were divided into Control group(blank control),si-NC group(negative control),si-NUTM2A-AS1 group(silence of NUTM2A-AS1)and si-NUTM2A-AS1+MAPK group(silence of NUTM2A-AS1 plus 50 nmol/L MAPK activator p79350).Cell counting kit-8(CCK-8),scratch and Transwell assays were used to evaluate cells'proliferation,migration and invasion,respectively.Western blot was applied to detect the expressions of p-ERK and p-MEK.Results Compared to BEAS-2B cells,NUTM2A-AS1 was high expressed in NSCLC cells(P＜0.01).Compared to si-NC group,there were weakened abilities of proliferation,migration and invasion as well as decreased expressions of p-ERK and p-MEK in si-NUTM2A-AS1 group(P＜0.05).In addition,the proliferation,migration and invasion abilities of A549 cells in si-NUTM2A-AS1+MAPK group were higher than those in si-NUTM2A-AS1 group in parallel with the enhanced expressions of p-ERK and p-MEK(P＜0.05).Conclusion NUTM2A-AS1 promoted proliferation,migration and invasion of NSCLC cells by activating the MAPK signaling pathway,which might provide a novel therapeutic target for NSCLC.
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