Effects of TRIM52-AS1 on migration,invasion and NF-κB signaling pathway of breast cancer cells by targeting microRNA-378a-3p
Objective To identify the role of TRIM52 antisense RNA 1(TRIM52-AS1)on proliferation,migration and invasion of breast cancer cells through targeting microRNA-378a-3p(miR-378a-3p).Methods Expression levels of TRIM52-AS1 and miR-378a-3p in breast cancer cells were detected by qPCR assay.BT474 cells were allocated into siRNA-NC group,siRNA-TRIM52-AS1 group(TRIM52-AS1 silencing)and siRNA-TRIM52-AS1+miR-378a-3p inhibitor group(TRIM52-AS1 and miR-378a-3p silencing).The proliferation,migration and invasion abilities of BT474 cells were accessed by MTT,scratch and Transwell assays.Dual luciferase assay was used for analysis of the relationship between TRIM52-AS1 and miR-378a-3p.Western blot was used to detect the phosphorylation levels of nuclear factor kappa B(NF-κB)p65 and inhibitory protein IkB alpha(IKB-α).Results TRIM52-AS1 was over-regulated in breast cancer cells(P＜0.05).Compared with the siRNA-NC group,the siRNA-TTRIM52-AS1 group showed reduced proliferation,migration,and invasion abilities,as well as decreased p-p65 level and increased p-IKBα level(P＜0.05).As a target gene of TRIM52-AS1,miR-378a-3p was negatively regulated by TRIM52-AS1.The siRNA-TTRIM52-AS1+miR-378a-3p inhibitor group showed enhanced abilities of cells'proliferation,migration,and invasion compared to the siRNA-TTRIM52-AS1 group,with decreased p-IKBα level and increased p-p65 level(P＜0.01).Conclusion TRIM52-AS1 promotes migration and invasion of breast cancer cells by targeting miR-378a-3p and regulates NF-κB signaling pathway.TRIM52-AS1 acts as a cancer driver and may serve as a potential therapeutic target for breast cancer.
Breast cancerTRIM52 antisense RNA 1MicroRNA-378a-3pNuclear factor kappa B signaling pathwayMigrationInvasion
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