Analysis of the expression and clinical significance of ECT2 gene in lung adenocarcinoma based on bioinformatics database
Objective To explore the expression and clinical prognostic significance of epithelium cell transforming sequence 2(ECT2)gene in lung adenocarcinoma based on bioinformatics database.Methods We obtained ECT2 gene expression data and clinicopathological characteristics parameters in lung adenocarcinoma tissues from bioinformatics databases including TCGA,GEO,and Sangerbox.Then we compared the differences in ECT2 expression in lung adenocarcinoma and adjacent tissues,analyzed the correlation of ECT2 expression with clinical characteristics and epidermal growth factor receptor(EGFR)mutation status,explored the significance of ECT2 as an independent prognostic factor to assess patient prognosis,mined the signaling pathways involved in ECT2 by gene set enrichment-analysis(GSEA),and assessed the correlation between ECT2 expression and the degree of immune cell infiltration in the microenvironment of lung adenocarcinoma using ESTIMATE algorithm.Results In the TCGA,GSE31210,GSE40791,GSE10072,GSE32863 and GSE75037 databases,the expression of ECT2 gene in lung adenocarcinoma tissues was significantly higher than that in adjacent tissues(P＜0.001).ECT2 expression was strongly correlated with gender,smoking status,T-stage,N-stage and TNM-stage,and was significantly lower in patients with EGFR mutation(P＜0.05).High ECT2 expression showed as an independent prognostic risk factor for lung adenocarcinoma patients,and the nomogram model combining T-stage and N-stage could more accurately predict the prognosis of patients.The GSEA analysis showed that patients with high ECT2 expression were enriched for activation of signaling pathways such as mTORC1 signaling,unfolded protein response,mitotic spindle,Myc targets,G2/M checkpoint,E2F targets and DNA repair.ESTIMATE algorithm results showed that ECT2 expression was correlated with stromal score,immune score,and with B cells,CD4+T cells,and neutrophil granulocyte infiltration(P＜0.05).Conclusion ECT2 is highly expressed in patients with lung adenocarcinoma and is an independent risk factor for malignant phenotype and poor prognosis,and is closely associated with EGFR mutation status and immune cell infiltration in the tumor microenvironment,which can be used as a potential biomarker for therapeutic screening and prognostic monitoring.
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