乳腺癌组织中SLC5A5的表达及与分子病理分型的关系分析
Expression of SLC5A5 in breast cancer tissues and its correlation with molecular pathological classification
于雷 1于晶晶 1薛汉杰 1贾亚男 2孟嫦娟3
作者信息
- 1. 054000 河北邢台 邢台市中心医院腺体外科
- 2. 054000 邢台市中心医院科教科
- 3. 054000 邢台市中心医院病理科
- 折叠
摘要
目的 探讨溶质载体家族 5A5(SLC5A5)在乳腺癌组织中的表达及与分子病理分型的相关性分析.方法 利用TGGA数据库分析SLC5A5 基因在乳腺癌组织中的表达,TIMER数据库分析SLC5A5 表达与乳腺癌预后的关系.采用免疫组化技术和qPCR实验检测 120 例乳腺癌患者的肿瘤组织和癌旁组织中SLC5A5 的表达水平,并分析SLC5A5 表达与乳腺癌临床病理学参数及病理分型的关系.结果 TGGA数据库在线分析结果发现SLC5A5 基因在乳腺组织中高表达(P＜0.05);乳腺癌组织中SLC5A5 累积光密度值高于癌旁组织(6.28±0.72 vs.2.54±0.34)、阳性率高于癌旁组织[65.8%(73/120)vs.34.2%(38/120)]、mRNA水平较癌旁组织升高(3.21±0.29 vs.1.03±0.15),上述差异均有统计学意义(P＜0.05).SLC5A5 表达与年龄、肿瘤直径和组织学分级无关(P＞0.05),而与淋巴结转移、肿瘤分期和分子病理分型有关(P＜0.05).生存分析显示SLC5A5 表达与乳腺癌总体及不同分子病理分型患者的生存期均无关(P＞0.05).结论 乳腺癌组织中SLC5A5 升高,且其表达与淋巴结转移、肿瘤分期和分子病理分型有关,在乳腺癌诊治中有一定指导价值.
Abstract
Objective To investigate the expression of solute carrier family 5A5(SLC5A5)in breast cancer tissues and the correlation with molecular pathological classification in breast cancer tissues.Methods TGGA database was used to analyze the expression of SLC5A5 gene in breast cancer tissues,and TIMER database was used to analyze the relationship between SLC5A5 expression and the prognosis of breast cancer.SLC5A5 expression in tumor tissues and adjacent tissues of 120 patients with breast cancer was detected by immunohistochemistry and qPCR,and relationship of SLC5A5 expression with clinicopathological parameters and pathological classification of breast cancer was analyzed.Results The online analysis of TGGA database showed that SLC5A5 gene was highly expressed in breast tissue(P＜0.05).The cumulative optical density of SLC5A5 in breast cancer tissues was higher than that in adjacent tissues(6.28±0.72 vs.2.54±0.34),and the positive rate was higher than that in adjacent tissues[65.8%(73/120)vs.34.2%(38/120)];quantitative results of qPCR showed that the level of SLC5A5 mRNA in breast cancer tissue was higher than that in adjacent tissue(3.21±0.29 vs.1.03±0.15),and the above difference was statistically significant(P＜0.05).The SLC5A5 expression was not significantly correlated with age,tumor diameter and histological grade(P＞0.05),but was related to lymph node metastasis,tumor staging and molecular pathological classification(P＜0.05).Survival analysis showed that the expression of SLC5A5 was not related to the survival time of breast cancer patients with different molecular pathological types(P＞0.05).Conclusion SLC5A5 is elevated in breast cancer tissue,and its expression is related to lymph node metastasis,tumor stage and molecular pathological classification,which has certain guiding value in the diagnosis and treatment of breast cancer.
关键词
乳腺癌/溶质载体家族5A5/分子病理分型/临床意义Key words
Breast cancer/Solute carrier family 5A5/Molecular pathological classification/Clinical significance引用本文复制引用
出版年
2024
NETL
NSTL
万方数据