Expression and clinical significance of GLDC in non-small cell lung cancer tissues
Objective To investigate the level and clinical significance of glycine decarboxylase(GLDC)in non-small cell lung cancer(NSCLC)tissues.Methods Real-time quantitative PCR(qPCR)was used to detect the levels of GLDC in 89 pairs of NSCLC tissues and adjacent tissues from Jan 2019 to Jun 2022.Relationship of tissue GLDC levels with clinicopathological characteristics and prognosis was stratified and analyzed.Cox proportional hazards model was used for multivariate analysis,and TIMER2.0 was used to evaluate the correlation between GLDC expression and tumor immune cell infiltration richness.Results The GLDC level in 89 NSCLC tissues was 4.362±1.450,higher than 1.343±0.406 in adjacent tissues(P<0.001).Stratified analysis revealed that the GLDC level in tissues was related to tumor maximum diameter,TNM staging,lymph node metastasis,and differentiation degree(P<0.05).Univariate analysis showed that tumor maximum diameter,TNM staging,lymph node metastasis,and GLDC level were associated with overall survival(OS)in NSCLC patients(P<0.05).Among them,the median OS of patients with low GLDC level was higher than 46 months of patients compared with high GLDC level.Multivariate analysis revealed that elevated level of GLDC was a risk factor for poor OS in NSCLC patients(HR=2.052,95%CI:1.518-2.776,P=0.002).The GLDC level in tissues was negatively correlated with the infiltration level of dendritic cells in lung adenocarcinoma(r=-0.198,P=9.44e-06),and negatively correlated with the infiltration level of macrophages in lung squamous cell carcinoma(r=-0.127,P=5.36e-03).Conclusion The abnormally high expression of GLDC in NSCLC tissues is involved in the malignant progression of the tumor and is associated with poor prognosis.It is related to dendritic cell infiltration in lung adenocarcinoma and macrophage infiltration in lung squamous cell carcinoma,and is expected to become a potential target for the prevention and treatment of NSCLC.
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